聚合物合金法制备神经生长因子缓释微球及其性能检测.doc

文档介绍：・ 5166・

中华临床医师杂志( 电子版)2012 年 9 月第 6 卷第 17 期 Chin J Clinicians( Electronic Edition ) ,September 1,2012 ,,
・论著・
聚合物合金法制备神经生长因子缓释微球
及其性能检测
刘华蔚温伟生孙华燕郑爱萍胡敏陈丽洁
【摘要】目的制备神经生长因子( nerve growth factor ,NGF) 缓释微球,并对其一般性质及体外释药性
溶
复乳法所制备微球进行比较,对所制备微球形态、粒径、包封率、累计释放率及体外释药性能等进行检测。
结果两种方法所制备微球大小形态符合实验要求,传统复乳法 NGF 缓释微球突释率(22畅3 ±1畅6 ) %、包封
率(23畅7 ±2畅1) %、累计释放率(78畅6 ±2畅0 ) %、可持续释放有活性 NGF 达 60 d;聚合物合金法 NGF 缓释微球
突释率(8畅8 ±0畅7) %、包封率(49畅5 ±3畅4 ) %、累计释放率( 91畅2 ±2畅2 ) %、可持续释放有活性 NGF 达 90 d,
两者突释率、包封率、累计释放率均有统计学差异。结论聚合物合金法较传统复乳法制备的 NGF 缓释微
球突释率低,包封率、累计释放率高,释放活性的 NGF 时间长,具有更稳定的体外释药性能。
【关键词】神经生长因子; 微球体; 药物释放系统
Preparation and in vitro drug release of nerve growth factor microspheres by polymer唱lloys method LIU
Hua唱ei,WEN Wei唱heng,SUN Hua唱an, ZHENG Ai唱ing, HU Min, CHEN of Stomatology, PLA
General Hospital,Beijing 100853,China
Corresponding author:WEN Wei唱heng,Email:wenws_pla@
【Abstract】 Objective To prepare the controlled release microspheres of nerve growth factor ( NGF) and its
general properties and drug release characteristics in vitro were studied .Methods The NGF microspheres were
obtained by polymer bined with oil 唱il emulsion /olvent evaporation ( S / / ) method, compare the
morphology ,particle size,encapsulation efficiency ,accumulative release rate and drug release characteristics in vitro
with the microspheres which made by traditional double em