文档介绍：华西口腔医学杂志第 27 卷第 4 期 2009 年 8 月
· 378 ·
West China Journal of Stomatology
[文章编号] 1000-1182(2009)04-0378-03
肿瘤坏死因子-α对大鼠成骨细胞
生长影响的实验研究
薛立伟1 张君1 王旭霞1 卜涛1 刘梅2
(; , 山东济南 250012)
[摘要]
目的
通过观察肿瘤坏死因子-α(TNF-α)对大鼠成骨细胞生长的影响,探讨TNF-α对成骨细胞的分子生
物学调控作用。方法
采用不同质量浓度TNF-α诱导大鼠成骨细胞,利用MTT法检测成骨细胞增殖的变化,利用4-
硝基苯基磷酸二钠盐(PNPP)偶氮法检测成骨细胞碱性磷酸酶(ALP)活性情况。结果
大鼠成骨细胞增殖活性及ALP
活性随TNF-α质量浓度的升高而降低,其中当TNF-α质量浓度大于50 ng/mL时,与对照组的差异有统计学意义(P<
)。结论 TNF-α对成骨细胞的增殖和分化均有抑制作用,当TNF-α质量浓度大于50 ng/mL时,这种抑制作用更
明显。
[关键词]
肿瘤坏死因子-α; 成骨细胞; 生长
[中图分类号]
R
[文献标志码]
A
Effects of tumor necrosis factor-α on the growth of rat osteoblasts
XUE Li-wei1, ZHANG Jun1, WANG Xu-
1 1 2
Jinan 250012, China; 2. Dept. of Stomatology, Qilu Hospital, Shandong University, Jinan 250012, China)
[Abstract]
Objective
To investigate the effects of tumor necrosis factor alpha(TNF-α) on the growth of rat os-
teoblasts. To find out the mechanisms that TNF-α regulates the growth of osteoblasts. MethoDs
To assay osteoblasts
proliferation by MTT. To assay alkaline phosphatase(ALP) activity of osteoblasts by PP-nitrophenyl phosphate(PNPP).
Results
The osteblasts proliferation and the ALP activity decreased in treatment groups, and the significantly lower
levels were observed in above 50 ng/mL groups(P<). Conclusion
TNF-α restrained osteoblasts prolifera