文档介绍：现代食品科技
Modern Food Science and Technology
2011, ,
香蕉炭疽菌拮抗生防菌株的筛选及鉴定
罗萍,区建发,杨炜钊,方祥
(华南农业大学食品学院生物工程系,广东广州510642)
摘要:从香蕉组织中分离筛选到 18株在平板上对香蕉炭疽菌孢子有明显抑菌活性的菌株,将这 18株菌分别与香蕉炭疽菌共同接
种到香蕉果指上进行活体防效复筛,发现与清水对照组相比较,AcT-02、AcT-07、AcT-09、AcT-11、AcT-13共 5株细菌均表现出显
著的防治效果,防效分别为 %±%、%±%、%±%、%±%和 %±%,香蕉病情指数显著降
低;将该 5株菌进行香蕉自然发病的防效试验,发现只有 AcT-13菌株具有显著防治效果,其病情指数为 %,比对照降低了 13%,
将 AcT-13进行形态学观察、生理生化特性测定以及分子学序列分析,初步将 AcT-13菌株鉴定为解淀粉芽孢杆菌 Bacillus
amyloliquefaciens。
关键词:香蕉炭疽菌;拮抗菌;生物防治
文章篇号:1673-9078(2011)3-275-278
Screening and Identification of anisms against Banana
ose Fungus
LUO Ping, OU Jian-fa, YANG Wei-zhao, FANG Xiang
(Department of Bioengineering Food College, South China Agricultural University, Guangzhou 510642, China)
Abstract: 18 Strains of antagonistic were isolated from plant tissue of banana, which showed strong square inhibition zone in plate
containing banana ose spore suspension. And then antagonistic experiments in vivo of banana by artificial infection with banana
ose and antagonistic strains were carried out. the result showed that, compared to the control of water, only AcT-02, AcT-07, AcT-09,
AcT-11, and AcT-13 could decrease banana ose, with their control effect being of %±%, %±%, %±%,
%±% and %± %, respectively. The disease index significantly discrease