文档介绍：2012-08-25
DOI: .
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应用与环境生物学报 Chin J Appl Environ Biol 2012,18 (4): 605~609
UV-B辐射下悬沙对小球藻生长和DNA损伤的影响*
李云1** 黄耀佳2 王晓蓉2
(1中国水产科学研究院东海水产研究所上海 200090)
(2华东师范大学生命科学学院上海 200062)
摘要
研究了UV-B辐射(17 µW cm-2,5 min/d)下不同浓度(0、100和1 000 mg L-1)和粒径组成(全粒径组和粒径< 38
µm组)的悬沙对小球藻(Chlorella sp.)生长和 DNA损伤的影响. 结果显示,小球藻的生长可用Logistic增长模型拟合,
拟合后的生长参数表明悬沙的“遮荫效应”减轻了UV-B辐射对小球藻细胞的DNA损伤,从而对小球藻生长产生正影
响. 悬沙浓度越高,UV-B辐射对小球藻细胞 DNA损伤程度越低,小球藻的环境负载能力a和瞬时增长率 K越大. 当悬沙
浓度为100 mg L -1时,全粒径组a值和 K值小于粒径< 38 µm组,且两组间小球藻细胞 DNA损伤差异显著(P < ),但
当悬沙浓度达1 000 mg L-1时,全粒径组a值和 K值大于粒径< 38 µm组,两组间细胞的DNA损伤无显著差异(P > ).
图3 表 2 参 24
关键词悬沙;UV-B辐射;小球藻;细胞密度;DNA损伤
CLC :
Effects of Suspended Solids on Growth and DNA Damage of Chlorella sp.
Exposed to UV-B Radiation*
LI Yun1**, HUANG Yaojia2 & WANG Xiaorong2
(1East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200090, China)
(2School of Life Sciences, East China Normal University, Shanghai 200062, China)
Abstract Effects of suspended solids (SS) with different concentrations (0, 100 and 1 000 mg L -1) and different particle sizes
(all size range and size < 38 µm) on the growth and DNA damage of Chlorella sp. exposed to UV-B radiation (17 µW cm-2, 5
min/d) were studied. The growth of Chlorella sp. was well predicted by a logistic model, and the growth parameters showed
that,