文档介绍：The change in eye temperature during the 30 min pre-treatment
period (excluding the first sample) was calculated by linear
regression for
each animal each day. These slopes were then
analysed using a two way ANOVA to calculate the mean change
for each treatment. The change in
eye temperature in response to
each treatment was calculated for each animal by subtracting the
average temperature during the 30 min pre
-treatment period from
the average temperature during 30 or 60 min post-treatment.
Changes in eye temperature were analysed using ANOVA to
determine the treatment effects. The same method was used for
core body temperatures except that a REML (restricted maximum
likelihood)
analysis was used because not all animals had core
body temperature measurements on all days. For the social
isolation treatment, changes
in eye temperature from pretreatment
to the first sample post-treatment and to 15 and
30 min post-treatment were calculated for each anima
l. The
significance of these differences was tested using a Student's t
test. The peak height, the time to peak and the adjusted area unde
r
the curve (treatment response area minus control response area,
AUC) for ACTH and cortisol concentrations were calculated for
each anim
al each day, and analysed using ANOVA. For ACTH,
both the AUC and the peak height were log transformed due to a
non-normal distribution of