文档介绍：血必净对内毒素休克大鼠心肌TNF-α变化的影响
陈洁,姜虹,朱也森
(上海交通大学医学院附属第九人民医院,200011)
目的本实验通过建立大鼠内毒素休克模型,旨在探讨血必净注射液对休克大鼠心肌细胞肿瘤坏死因子(TNF-α)变化的影响,为临床脓毒症休克的复苏治疗提供相关依据。
方法本研究选用Wistar成年雄性大鼠65只,随机分为四组,即正常组(5只)、模型组(20只)、治疗组Ⅰ(20只)和治疗组Ⅱ(20只);采用腹腔注射大肠杆菌脂多糖(LPS)方法制备大鼠内毒素休克模型,除正常组外,其余三组均腹腔注射LPS15mg/kg,注射完毕后即刻内毒素组大鼠予以皮下注射生理盐水10ml/kg抗休克治疗,而治疗组1和治疗组2则分别皮下注射血必净注射液5ml/kg+生理盐水5ml/kg和10ml/kg,正常组大鼠经腹腔注入等量生理盐水。除正常组外其余四组大鼠按不同时相(腹腔注射后30min、2h、6h)分为3个亚组,在不同时相点留取股静脉血标本以及心室肌标本,通过ELISA法测定TNF-α值。
结果内毒素组大鼠静脉血标本于30min时间点出现TNF-α值高峰,2h和6h时有所下降,其中30min的TNF-α值与正常组及对照组相比存在明显差异(P<)。
结论 ,导致TNF-α的分泌高峰出现。。-α的分泌和表达,减轻心脏本身的炎症反应。
关键词血必净注射液,内毒素休克,肿瘤坏死因子(TNF-α)
EFFECT OF XUEBIJING ON TNF-α OF CARDIAC MUSCLE IN
ENDOTOXIN SHOCK RATS
ABSTRACT
Objective By producing the rat models with endotoxin shock,to explore the effects of XUEBIJING on the changes of TNF-αof cardiac myocytes in endotoxin shock rats and offer a relevant evident on resuscitation treatment of endotoxin shock.
Methods The study choose sixty-five male Wistar rats randomized into four groups:Normal group(n=5);Experimental group(n=15);Therapeutic groupⅠ(n=15);Therapeutic groupⅡ(n=15).Endotonxin shock model was performed by intraperitoneal injection with group and Therapeutic group Ⅰand Ⅱ were injected with LPS(15mg/kg) through peritoneal cavity .Experimental group were injected sali