文档介绍：骨髓瘤细胞裂解物致敏的DC—CIK细胞抗骨髓瘤细胞作用
罗云,张萍#,白凤霞,娄世锋*
(重庆医科大学附属第二医院血液科, 重庆 400010)
摘要:目的研究负载骨髓瘤细胞裂解物的健康供者树突状细胞(dendritic cells,DCs)联合细胞因子诱导杀伤细胞(cytokine induced kil1er cells,CIK)体外对骨髓瘤U266细胞杀伤作用的影响。方法通过血细胞分离机采集健康供者单个核细胞100毫升用于体外培养,选取贴壁细胞进行DC细胞培养,悬浮细胞用于CIK细胞培养,DC细胞培养7日后用骨髓瘤U266细胞裂解物致敏,然后与CIK细胞共培养7日,用倒置显微镜、扫描电镜观察细胞形态,流式细胞仪检测细胞表型,乳酸脱氢酶释放法检测共培养细胞对U266细胞杀伤作用。结果 CIK细胞大部分形态为圆形,少数梭形,DC细胞有典型细胞突起,流式细胞仪分析培养的CIK细胞高表达CD3;CD3和CD56共表达细胞明显升高,在致敏后DC细胞表达CD86、CD80,共培养细胞对U266细胞杀伤作用强于单独CIK细胞作用。结论瘤细胞裂解物致敏的健康供者DC—CIK细胞能显著提高对瘤细胞的杀伤性,如在患者干细胞移植后应用可清除残留病变,值得进一步研究应用。
关键词:骨髓瘤树突状细胞免疫疗法过继细胞因子诱导杀伤细胞
Anti-myeloma cell effect by dentritic cells loaded with antigens of myeloma bined with cytokine induced kil1er cells
Luo Yun, Bai Feng-Xia, Zhang Ping et al
(Department of Hematology, the Second College of Clinical Medicine, Chongqing Medical University, Chongqing, 400010 China)
[Abstract] Objective This study was aimed to investigate the effect of donors cytokine induced killer cells bined with DCs loaded with whole antigens of U266 cells on the U266 cells survival in vitro. Methods 100mL Peripheral blood mononuclear cells were collected from healthy donor by hemapheresis. Adherent cells were cultured for DCs and suspension cells for CIK cells. After cultured for 7 days, DCs were loaded with whole antigens of U266 cells and CIK cells were added to co-culture. Inverted microscope and scanning electron microscope wer