三七粉中总黄酮的检测.doc

文档介绍：三七粉中总黄酮的检测
李忠英,罗跃中
(湖南化工职业技术学院湖南株洲 412004)
摘要:建立以芦丁为标准品三七粉中总黄酮含量检测方法。采用50%乙醇浸取+超声提取,用(亚硝酸钠—硝酸铝)紫外分光光度法测定其含量。以芦丁为对照标准品,确定了芦丁的最大吸收波长为510nm,以芦丁的吸光度和浓度制作标准曲线,得回归方程:y = - ,R2 = ,~;%,%。该法操作简便,结果准确,重复性好,为三七粉中黄酮含量的测定提供了一种切实可行的途径。
关键词:三七粉;超声波;总黄酮;分光光度法;检测
Determination of Total Flavanoids In Panax pseudo-ginseng
LI Zhong-Ying , LUO Yue-Zhong
(Hunan Chemical Industry Vocation Technology Institute , Zhuzhou HuNan 412004 China )
Abstract:Did establish a method which acted rutin as standard sample to measure determination of totalflavonoids in Panax pseudo- the 50%ethyl alcohol leaching and Ultrasonic Extraction , Determines its content with (sodium nitrite-aluminum nitrate) the UV spectrophotometry. Take grass cutter parison standard Had determined the maximum absorption wavelength of 510nm,Make the standard curve by the Rutin’s Absorbance and concentration,Regression equation y = - ,R2 = , Sample at a concentration of ~ The linear range;Determinationof total flavonoids in Panax notoginseng powder was3.36%,Canadian recovery was %,RSD is % .The method is simple, accurate, reproducible, and for the Panax pseudo-ginseng. content of powder provides a practical way.
Key words: Panax pseudo-ginseng;Ultrasonic;Total flavonoids;Spectrophotometry;Determination; De