文档介绍：实验十一DNA的酶切RestrictionEndonucleases: AnOverviewRestrictionenzymeswerediscoveredabout30yearsagoduringinvestigationsintothephenomenonofhost-,andthis"restriction""restrictionenzymes"tobepurifiedwereEcoR IandEcoRIIfromEscherichiacoli,,generatingdiscrete,gene-sizefragmentsthatcouldbere-,’,--allfree-;theyrangeinsizefromthediminutivePvuII(157aminoacids)tothegiantCjeI(1250aminoacids),000activitiesthathavebeenpurifiedandcharacterized,morethan250differentsequence-,over30%,bothbiochemically,bytheanalysisofcell-extracts,putationally,--isoschizomers--ofexistingspecificities,’s,,anditprovidesthegenesforsequencingandanalysis,andtheproteinsforx-,