文档介绍：高效液相色谱法测定甲苯磺酸妥舒沙星片的含量
石文宏朱庆玲
(内蒙古食品药品检验所呼和浩特 010010 )
摘要目的:建立甲苯磺酸妥舒沙星片含量测定的高效液相色谱法,方法:采用高效液相色谱法,C18柱(250×,5μm),()-乙腈(73:27),流速:,检测波长:270nm。结果:甲苯磺酸妥舒沙星在5~60ug/ml范围内与峰面积呈良好的线性关系(r =),%,%。结论:高效液相色谱法简便、灵敏、准确、重现性好,可用于测定甲苯磺酸妥舒沙星片含量。
关键词甲苯磺酸妥舒沙星片;高效液相色谱法;含量测定
ABSTRACT:OBJECTIVE To establish a HPLC method for concurrent determination of tab Tosufloxacin toluene sulfonate. METHODS The HPLC system consisted DiamonsilTM C18 column (×250mm,5µm), Citric acid(Triethylamine pH )-Acetonitrile (73:27) as mobile phase with detection wave Length at 270nm,flow rate was -1. , temprature of column was 25℃. RESULTS The linear range of Tosufloxacin toluene sulfonate were within5~60µg·mL-1 ,the average recorvery was %(RSD=%). CONCLUSION The established method was suitable for the concurrent determination of Tosufloxacin toluene sulfonate. The method was convenient and accurate.
KEY WORDS : HPLC;Tosufloxacin toluene sulfonate l ;
甲苯磺酸妥舒沙星片是新型广谱抗菌的喹诺酮类药物,其含量测定方法为紫外-分光光度法[1],本文应用反高效液相色谱法测定了甲苯磺酸妥舒沙星片的含量,获得了满意的结果。
1 仪器与试药
Agilent-1100高效液相色谱仪(美国);UV-265紫外分光光度计(日本)。
甲苯磺酸妥舒沙星对照品(赤峰万泽制药有限责任公司);甲苯磺酸妥舒沙星片(赤峰万泽制药有限责任公司);乙腈(天津市协和昊鹏色谱科技有限公司)
2 方法与结果
色谱条件色谱柱:DiamonsilTM(钻石) C18柱(250mm×,5μm);流动相:(用三乙胺调节pH至 )-乙腈(73:27);流速:;柱温:25℃;检测波长:270nm;进样量:20μl;理论板数按甲苯磺酸妥舒沙星计算应不低于2