文档介绍：玉竹中新高异黄酮类化合物及其生物活性研究
李丽红*,任风芝,陈书红,郑智慧,高月麒,张华,贺建功(华北制药集团新药研究开发有限责任公司天然药物室,河北石家庄 050015)
摘要:目的研究玉竹的生物活性成分。方法利用各种柱色谱及高压液相色谱等方法进行分离和纯化,根据理化性质和光谱数据对化合物结构进行鉴定;并应用高通量筛选模型对化合物进行评价。结果从玉竹中分离得到三个高异黄酮类化合物,确定结构分别为5,7-dihydroxy-6-methoxyl-8-methyl-3-(2′,4′-dihydroxybenzyl)chroman-4-one(Ⅰ),5,7- dihydroxy-6-methyl-3-(2′,4′-dihydroxybenzyl)- chroman-4-one(II),5, 7-dihydroxy- 6-methoxyl-8-methyl-3-(4′-methoxybenzyl)chroman- 4-one(Ⅲ), 三个化合物均显示了较强的抗肿瘤活性和抗炎活性。结论化合物I~III为未见文献报道的新化合物,其抗肿瘤和抗炎活性为首次报道。
关键词:玉竹;高异黄酮;化学成分;抗肿瘤;抗炎;人白细胞弹性蛋白酶
Study on the new homoisoflavonoids and their bioactivity from Polygonatum odoratum (Mill.)Druce
LI Li-hong*, REN Feng-zhi, CHEN Shu-hong, ZHENG Zhi-hui, GAO Yue-qi, ZHANG Hua, HE Jian-gong(Department of Natural Product Drug Discovery, Shijiazhuang 050015)
ABSTRACT: OBJECTIVE To study the bioactive constituents of Polygonatum odoratum (Mill.)Druce. METHODS pounds were separated by column chromatography and HPLC. On the basis of physicochemical properties and spectral data , their structures were confirmed. High through-put screening method was used to explore the activity of pounds. RESULTS pounds were isolated and identified as 5,7-dihydroxy-6methoxyl-8-methyl-3-(2′,4′-dihydrox-ybenzyl)chroman-4-one (Ⅰ), 5,7-dihydroxy-6-methyl-3-(2′,4′-dihydroxybenzyl)-chroman-4-one (II), 5, 7-dihydroxy-6-methoxyl-8- methyl-3-(4′-methoxybenzyl)chroman-4-one (Ⅲ). pounds showed signi