文档介绍：水苏糖对猪精液冷冻保存效果的影响
杨升1,贾东平1,刘家慧2
(,天津 300384;,青岛 266555)
摘要:为了提高猪精液冷冻保存效果,试验采用在传统的ZO稀释液基础上分别添加1%、2%、4%、8%的水苏糖的方法,研究不同浓度水苏糖对猪精液冷冻后精子质量的影响。结果表明,水苏糖相对于对照ZO稀释液能够显著改善和提高猪精液的冷冻效果,其最佳添加浓度为4%,冷冻-解冻后猪精子活力、活率、质膜完整性以及顶体完整率均显著提高,水苏糖可以明显抑制精子获能,%,%,有利于抑制精子获能,精液稀释液中甘油的适宜添加浓度为3%,说明水苏糖与甘油共同作用能在冷冻-解冻过程更加有效地保护精子。
关键词:猪;精液;水苏糖;冷冻
Abstract:In traditional ZO diluent basis, respectively add 1%, 2% , 4%, 8% of the stachyose, research different concentrations of pig sperm water stachyose the influence of frozen sperm quality. The results show that, stachyose ZO diluent relative to contrast to significantly improve and enhance the pig sperm freezing effect, Its best dosage is 4%, Frozen-defrost pig sperm motility, live rate, plasma membrane integrity intact rate and the body to be significantly improved. stachyose can significantly inhibit sperm get energy, former capacitation won can handle only, can a %.To gain can handle percent, after, to restrain sperm get energy. Sperm in the appropriate dilutions add concentration of glycerol 3%. stachyose and mon role,can freeze-thawing process more effectively protect sperm.
Key words: sperm;semen;stachyose;frozen
自1956年Polge首次利用冷冻精液人工授精获得仔猪至今,猪精液冷冻技术虽然经过多年的发展,但与奶牛冷冻精液的应用相比,仍有很大差距。早在1930年,Milovanov等对猪人工授精进行了试验,并提出最初的猪精液稀释剂(葡萄糖-硫酸盐和葡萄糖-酒石酸盐) 。近年来猪精液冷冻保存液中非渗透性保护剂糖类物质的加入引起了研究人员的高度关注,糖类在生物活性细胞脱水的情况下在细胞膜外形成一层保