文档介绍：siRNA 沉默人类血红素敏感基因1对膀胱癌T24细胞的影响
汤磊1 李瑞晓于垂恭袁建林武国军#
汤磊:第四军医大学,西京医院泌尿外科,硕士研究生,710032,
email:tanglxian@
李瑞晓:第四军医大学,西京医院泌尿外科,硕士研究生,710032
于垂恭:第四军医大学,西京医院泌尿外科,博士研究生,710032
袁建林:第四军医大学,西京医院泌尿外科,医学博士,科室主任,博士生导师,710032
武国军:第四军医大学,西京医院泌尿外科,医学博士,科室副主任,硕士生导师,710032 email:liruixiao4@
siRNA 沉默人类血红素敏感基因1对膀胱癌T24细胞的影响
汤磊1 李瑞晓于垂恭袁建林武国军#
1 第四军医大学西京医院泌尿外科,710032 (tanglxian@)
# 第四军医大学西京医院泌尿外科,710032(liruixiao4@)
摘要目的观察人类血红素敏感基因1(HRG-1)基因在膀胱正常组织和膀胱肿瘤组织中的表达情况,通过RNA干扰技术抑制HRG-1在膀胱癌T24细胞表达水平,观察生长增殖的变化。方法应用免疫组化SP法检测85例膀胱肿瘤(膀胱乳头状瘤25,低级别膀胱癌30,高级别膀胱癌30)和20例膀胱正常组织石蜡标本中HRG-1表达情况,并进行相关临床病理分析。设计针对HRG-1基因的siRNA,转染膀胱癌T24细胞,应用免疫印迹法和RT-PCR分析HRG-1 siRNA的表达,MTT和流式细胞术(FCM)检测增殖凋亡的变化。结果正常膀胱组织和膀胱癌组织中HRG-1表达有显著性差异(P<);HRG-1的阳性表达与膀胱肿瘤病例分级、临床分期之间有显著性差异(P<);转染siRNA后,HRG-1表达水平下降,MTT和FCM检测发现,细胞增殖曲线受到抑制,HRG-1-siRNA组与空载体转染组及未转染组比较,细胞凋亡比例增加,差异均具有统计学意义(p<)。结论 HRG-1基因可能参与了膀胱癌的发病,siRNA-HRG-1处理T24细胞后,细胞的增殖受到抑制,同时凋亡比例增加。
关键词:膀胱肿瘤,人类血红素敏感基因,RNA干扰基因,细胞凋亡
siRNA Interference of HRG-1 on bladder cancer T24 in vitro
TANG Lei*,LI Rui-xiao YU Chui-gong,YUAN Jian-lin, WU Guo-jun,et a1.
* Department of Urology,Xijing Hospital,the Fourth Military Medical University,Xi′an 710032,China
Corresponding author:Wu Guo-jun (email:liruixiao4@)
Abstract Objective To observe the HRG-1 gene expression in carcinoma of urinary bladder and normal urinary bladder tissues,and to investigate the effect of HRG-1-siRNA on the proliferation and apoptosis of human bladder cancer cell line. Methods Immunohistochemisty was used to detect the expression of HRG-1 in 85 cases of bladder carcinoma and 20 normal bladder tissues, and The siRNA of HRG-1 was designed, synthesized and transfected into bladder cancer cell line T24. Results The HRG-1 gene expression had significant differences between bladder carcinoma and normal bladder tissues (p<); HRG-1 gene positive expression had marked differences between the pathological grades and clinical stages of bladder carcinoma (p<). After treated with siRNA, the expression level o