文档介绍：正交实验法优选沙枣花总黄***提取工艺及沙枣花醇提物体外抗氧化活性的研究
作者:王基云,陆钊罡,王丹,肖旭,张振华,姚遥,王锐,王妍
【摘要】目的研究提取沙枣花(elaeagnus angustifolia blossoms,EAB )中总黄***的最佳工艺条件,并观察了沙枣花醇提物(the alcohol extracts of elaeagnus angustifolia blossoms,AE-EAB )清除氧自由基的作用。方法以芦丁为对照品,NaNO2- Al(NO3)3-NaOH显色体系作为显色剂,采用正交实验方法对沙枣花总黄***提取方法进行了系统研究。结果最佳提取工艺为20倍量70%乙醇回流3次, 1 h/次。AE-EAB对氧自由基的清除作用随着浓度的升高而增强。结论EAB总黄***的最佳提取工艺为A2B2C1D3。AE-EAB对氧自由基有清除作用.
【关键词】沙枣花;总黄***;紫外分光光度法;正交实验; 氧自由基
Abstract:ObjectiveTo study the optimal extraction technology of total flavonoids in Elaeagnus angustifolia blossoms( EAB),and the free radicals scavenging effect of the alcohol extract of Elaeagnus angustifolia blossoms(AE-EAB ) .MethodsUsing Rutin as the evaluating criteria and NaNO2- Al(NO3)3-NaOH as chromogenic agent, the extraction condition was studied by orthogonal optimal technological conditions were attained by the orthogonal experiment as follows: extraction with70% ethanol, ethanol volume 20 ml for 1 g EAB every time, and extracting for 3 times (4
0 min for each time).The scavenging effect on oxygen free radical was proportional to the concentration of AE- optimum extraction technology is A2B2C1D3 . AE-EAB has scavenging effect on oxygen free radical .
Key words:Elaeagnus an gustifolia L .;Total flavonoid;UV;Orthogonal design
沙枣花为胡颓子科植物沙枣 Elaeagnusan-gustifolia L .的花,主产于西北地区,在宁夏地区资源非常丰富。据《中药大辞典》记载,沙枣花主要含山萘酚、花白素、脂肪油和少