文档介绍：免疫学
Rapid Acquisition of Tissue-specific
Homing Phenotypesby CD4+ T Cells
ActivatedIn Cutaneous or Mucosal
Lymphoid Tissues
专业:临床医学
班级:五班
年级:2009级
本组成员:

韩佳彤
刘文琪
刘侠
牛亚运
宋瑞珍
王志远
杨雪
赵贵苓
岳红梅
庄敏丽
Keywords:
T cell, homing, chemokine, adhesion,
lymph node
Introduction
To maximize the chances of antigen encounter, naive lymphocytes
recirculate through specialized secondary ans
such as the spleen, lymph nodes, and Peyer's patches (PP),
which efficiently trap, concentrate, and present antigens.
However, effector lymphocytes often function in nonlymphoid sites
of inflammation and infection. Moreover, effector and
memory lymphocytes generally display selective tropism for
specific peripheraltissues such as the skin or
intestinal lamina propria
Materials and Methods
Mice and Adoptive Transfers.
BALB/c and −/− mice were bred and housed
at the Veterans Administration Hospital in Palo Alto, CA.
Mice used in experiments shown in Figs. 1 and and22 were
>1 y old. For all other experiments, mice were 1–3 mo old.
BALB/c micewere given between 5–10 × 106 erythrocyte-depleted
splenocytesfrom sex-matched -2−/− animals by
retroorbital injection 24 h before immunization.
For 5-(and-6)-carboxyfluorescein diacetate,
inimidyl ester (CFSE) labeling, cells were
incubated for 10 min at 37°C in HBSS
(5 × 106 cells per milliliter) containing 500
nM CFSE (Molecular Probes).
Figure 1.
α4β7 and P-lig define three CD4+ memory T
cell populations that differentially localize in intestinal
and cutaneous ans. (Left) CD4 and CD45RB
expression by lymphocytes isolated from the cutaneous
PLNs of a >1-y-old BALB/c mouse. The gates used to define
‘naive’(CD4+CD45RB+) and ‘memory’(CD4+CD45RB−) T
Cells are shown. (Right) α4β7 and P-lig expression by
Gated Naïve and memory CD4+ T cells isolated from the
indicated lymphoidtissues of a >1-y-old BALB/c mouse.