文档介绍：南方红豆杉水提物对人肺癌A549细胞增殖/抑制作用的研究
舒琦瑾1﹡李萍2 王彬彬1 朱国猛1
(1浙江中医药大学附属第一临床医学院肿瘤科,杭州310006;2杭州师范大学附属医院肿瘤科,杭州310015)
摘要:目的研究南方红豆杉水提物对人肺癌A549细胞增殖的抑制作用和机制。方法采用MTT法检测南方红豆杉水提物对A549细胞增殖的抑制作用;生长曲线法观察其抑制A549细胞的时间-效应曲线;流式细胞仪检测其对A549细胞周期及凋亡的影响;倒置显微镜及电镜观察其对A549细胞形态的影响。结果南方红豆杉水提物对A549细胞的增殖有抑制作用;其抑制作用机制为使A549细胞周期出现明显的G0/G1期阻滞,诱导细胞凋亡。结论南方红豆杉水提物对人肺癌A549细胞的增殖有抑制作用。
主题词:南方红豆杉;抑制作用;人肺癌A549细胞;细胞增殖
Study on Inhibitory Action of Proliferation of human lung carcinoma A549 Cell with aqueous extract of Taxus chinensis
Shu Qi-jin1﹡, Li Ping2, Wang Bin-bin1, Zhu Guo-meng1
of Oncology,the First Affiliated Hospital,Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, China 2. Department of Oncology,the Affiliated Hospital of Hangzhou Normol University Hangzhou 310015, China
Corresonding author: Shu Qi-jin, E-mail: ******@.
Abstract:Objective To study the inhibitory effect of aqueous extract of var on vitro cultured human lung carcinoma A549 cell, and to explore its The inhibitory effect of var on human lung carcinoma A549 cell was tested by MTT method; time-effect relationship was observed by growth curve;effects on blockage of cell cycle and apoptosis was inspected by flow cytometry; morphology of the A549 cell was observed by inversion microscope and electron microscope. Results The aqueous extract of var has inhibitory e