文档介绍：咪达唑仑对新生大鼠离体脊髓运动神经元的作用
基金项目:安徽省自然科学基金(090413084)
作者简介:宦才娟(1981-),女, 2008级硕士研究生, (电话) **********,(电子邮箱)hcj520 @;汪萌芽,男,教授,博士,硕士生导师, *通讯作者(E-mail: ******@wnmc.)
宦才娟,汪萌芽*
(皖南医学院细胞电生理研究室,安徽芜湖241002)
Effects of midazolam on motoneurons in neonatal rat spinal cord in vitro
HUAN Cai-juan, WANG Meng-ya*
Cell Electrophysiology Laboratory, Wannan Medical College, Wuhu, Anhui 241002, China
[Abstract] Objective: To observe the effects of midazolam on motoneurons (MNs) in neonatal rat spinal cord slices. Methods: The conventional intracellular recording techniques were used to observe the effects of midazolam at distinct concentration on MNs of spinal cord slices isolated from neonatal rats (8~14d old). Results: In the 5 MNs steadily recorded, not only a depolarization response of (±) mV with increase of membrane resistance was induced by perfusion of midazolam at - mmol/L for 15 min of each concentration (, P< or P<), but also amplitude of action potentials was decreased (P<), and even action potentials were nullified in 2 MNs. In 4 MNs with dorsal rootlet stimulation-evoked excitatory postsynaptic potentials (DR-EPSPs), bath of midazolam (- mmol/L) reversibly depressed DR-EPSPs in a concentration-dependent manner. Moreover, in 3 MNs with DR-EPSP followed by inhibitory postsynaptic potentials (DR-IPSPs), DR-EPSPs were inhibited and DR-IPSPs were enha