磷脂酶D 高效产生菌的筛选及鉴定.doc

文档介绍：磷脂酶D高效产生菌的筛选及鉴定
代书玲1 张江1 商军2
(1. 上海农林职业技术学院,上海松江:201600;2. 上海市兽药饲料检测所,上海:201103)
摘要:采用蛋黄平板法,从河南某香油作坊附近土样中筛选到两株降解磷脂的菌株X2和X10,产物分析和酶活测定表明,X2所产磷脂酶主要为磷脂酶D,,X10除产少量磷脂酶D外,可能还产磷脂酶A。根据形态观察、生理生化特征和BIOLOG自动微生物分析系统的鉴定,初步判定X2为蜡状芽孢杆菌(Bacillus cereus),X10为嗜麦芽糖寡养单胞菌(Stenotrophomonas maltophilia)。
关键词:磷脂酶D;菌株筛选;鉴定
Screening and Identification of the High Phospholipase D-producing Strains
Dai Shuling1,Zhang Jiang1,Shang Jun2
(1. Shanghai Vocational and Technical College of Agriculture and Forestry, Songjiang, Shanghai, 201600;2. Shanghai Institute for Veterinary Drug & Feeds control, Shanghai, 201103)
Abstract:The research aimed to screen and identify high producing strains of Phospholipase D. Strains were isolated from soil near a sesame oil workshop in Henan by the method of egg yolk plate and identified according to their shapes, physiological and biochemical characteristics, as well as BIOLOG automatic microbiology analysis system. Two degradating phospholipids strains X2and X10 were obtained. The analyses of the degradation products and Phospholipase D activity showed that strain X2 mainly produced phospholipase D and the activity was up to . while X10 produced other phospholipase in addition to a small amount of phospholipase D. According to the result of identification strainsX2 and X10 were Preliminarily identified as Bacillus cereus and Stenotrophomonas maltophi