文档介绍：HPLC-ELSD 法测定玉屏风口服液中黄芪甲苷的含量王建舫,张倩,穆祥*(北京农学院兽医学(中医药)北京市重点实验室,北京 102206)摘要[目的] 建立玉屏风口服液中黄芪甲苷的含量测定方法。[方法] 采用 HPLC-ELSD法,Venusil MP 色谱柱( mm×150 mm,5μm),乙腈-水(35∶65)为流动相,ELSD 漂移管温度 65 ℃,载气 N2,压力 30 psi。[结果] 黄芪甲苷在 ~ μg 范围内呈良好的线性关系,r = (n = 6)。平均回收率 %,RSD 为 %。[结论] 该法准确可靠,可用于玉屏风口服液的质量控制。关键词高效液相色谱; 蒸发光散射检测器;玉屏风口服液;黄芪甲苷基金项目北京市教育委员会科技计划面上项目(KM201410020007)。作者简介王建舫(1969-),女,河北肃宁人,实验师,博士,主要从事中药防治动物疾病的研究工作。* 通讯作者,教授,硕士生导师,E-mail: wjfhlx@。收稿日期 2014-12-09修回日期 2015-01-19Determination of Astragaloside lV in YupingfengOral Solution by HPLC-ELSD MethodJianfang WANG, Qian ZHANG, Xiang MU*Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, ChinaSupported by General Program of Science and Technology Plan of Beijing mission of Educational (KM201410020007).*Corresponding author. E-mail: ******@Received: December 9, 2014 Accepted: January 19, 2015AAgricultural Science & Technology, 2015, 16(2): 197-199Copyright 訫 2015, lnformation lnstitute of HAAS. All rights reserved Agricultural Basic Science and TechnologyYupingfeng oral solution con-sists of Astragalus mem-branaceus, Saposhnikovia di-varicata and Atractylodes macro-cephala. This formula has beenrecorded in "Dan Xi Xin Fa" in YuanDynasty. Yupingfeng oral solution hasfunctions of invigorating qi for consoli-dating superficies and preventing per-spiring[1-2]. Astr