文档介绍：Bioresource Technology 88 (2003) 103–106
Optimization of lipase-catalyzed biodiesel by response
surface methodology
C.-J. Shieh *, H.-F. Liao, C.-C. Lee
Department of Bioindustry Biotechnology, Dayeh University, 112 Shan-Jiau Road, Da-Tsuen, Chang-Hua 51505, Taiwan
Received 2 October 2002; received in revised form 8 November 2002; accepted 11 November 2002
Abstract
Biodiesel prepared by catalyzed mild transesterification has e of much current interest for bioenergy. The ability of a
commercial immobilized lipase (Novo Industries––Bagsvaerd, Denmark) from Rhizomucor miehei (Lipozyme IM-77) to catalyze the
transesterification of soybean oil and methanol was investigated in this study. Response surface methodology and 5-level-5-factor
posite rotatable design were employed to evaluate the effects on reaction time, temperature, enzyme amount, molar ratio of
methanol to soybean oil, and added water content on percentage weight conversion to soybean oil methyl ester by transesterification.
Based on ridge max analysis, the optimum synthesis conditions giving % weight conversion were: reaction time h, temperature
°C, enzyme amount BAUN (Batch Acidolysis Units NOVO), substrate molar ratio :1, and added water %.
Ó 2002 Elsevier Science Ltd. All rights reserved.
Keywords: Enzymatic biodiesel; Immobilized lipase; Optimization; Response surface; Transe