文档介绍：一株银杏内生真菌的鉴定及其活性代谢产物研究
王国平2,3#,王丽薇1,3#,张亚磊3,4,王佳莹2,3,徐旭辉5,
基金项目:公益性行业(农业)科研专项(200903052);杭州市创业种子基金(20102431K156)
作者简介:#对本文有同等贡献,王国平(1974-),男,本科,工程师,Tel: 0571-64156863;E-mail: yuiop380@;王丽薇(1976-),女,博士,讲师,E-mail:wangliwei78@。
通迅作者:章初龙,男,博士,副教授,Tel: 0571-86971185; E-mail:******@zju.
(,杭州 310036;,杭州 310029;,杭州 310029;,杭州 310029;5. 浙江大学农药与环境毒理研究所,杭州 310029)
摘要:药用植物内生真菌是一类重要的微生物资源,能代谢产生多种生物活性物质,本研究从浙江天目山自然保护区银杏Ginkgo biloba中分离获得1株活性菌株。通过形态学和ITS rDNA序列分析,mune。发酵粗提物采用正相硅胶柱层析和Sephadex LH-20凝胶柱层析,以紫外光或碘蒸汽显迹,配合活性追踪。从菌株TMSF169发酵液中分离获得1个具抗真菌及除草活性的化合物TMSF169A,通过质谱和核磁共振波谱等技术将其结构鉴定为圆弧菌醛酸(cyclopaldic acid)。活性测试表明,该化合物具有抗菌活性,melina suffruticosa),反枝苋(Amaranthus retroflexus),马唐(Digitaria sanguinalis)、空心莲子草(Alligator Alternanthera)、水稻(Oriza sativa L.)等植物均具有致病毒性,对芝麻(Sesamum indicum)和大豆(Glycine max)不敏感,该菌株具有较好的开发应用价值。
关键词:内生真菌;团青霉;活性代谢产物;除草活性;圆弧菌醛酸
中图分类号:,, 文献标识码:A
Identification of an endophytic fungus of Ginkgo biloba TMSF169 and its antifungal metabolites
Wang Guoping 2,3#, Wang Liwei 1,3#,Zhang YaLei3,4 , Wang JiaYing 2,3, Xu Xuhui5, Zhang Chulong 1,2*
1. College of Medicine and Public Health Management, Hangzhou Normal University, Hangzhou 310036; 2. Zhejiang Dahua Zhejiang University Biomedicament R&D Center, Hangzhou 311616; 3. Institute of Biotechnology, Zhejiang University, Hangzhou 310029;4. College of Pharmaceutical science, Zhejiang University of Technology,Hangzhou 310029; 5. Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China
Abstract: Endophytic fungi in medicinal plants are important sources of anisms, which produce a variety of pounds. In this study, an endophytic fungus TMSF169 with antifungal activity was isolated from Ginkgo biloba in Tianmushan Nature Reserve, Zhejiang. Strain TMSF169 was identified as mune based on the morphology and ITS rDNA sequence analysis. Antifungal and pounds were isolated from the fermentation broth o