文档介绍：不同β受体阻滞剂抗家兔动脉粥样硬化作用的研究
【摘要】目的探讨卡维地洛和不同剂量美托洛尔的抗动脉粥样硬化作用及其机制,并对二者的疗效作一对比。方法雄性新西兰大耳白兔40只,随机分为正常组、高脂组、小剂量美托洛尔组、大剂量美托洛尔组、卡维地洛组,每组8只,分别按不同方法喂养10周。实验结束后,分别检测各组家兔血清超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量。采用免疫组化的方法分别测定各组血管壁增殖细胞核抗原(PCNA)及核因子kBp65(NFkBp65)的表达。结果与正常组比较,高脂组血清SOD活性降低,MDA含量增加,差异有显著性(F=、,q=、,P<);高脂组,小、大剂量美托洛尔组血清SOD活性和MDA含量比较差异无统计学意义(q=~,P>);与高脂组及小、大剂量美托洛尔组比较,卡维地洛组血清SOD活性明显升高,血清MDA含量明显下降(q=~,P<)。A和NFkBp65的表达较正常组明显增加(F=、,q=、,P<);高脂组及小、大剂量美托洛尔组、A和NFkBp65的表达依次减少,差异有显著性(q=~,P<)。相关分析表明,A和NFkBp65水平呈正相关(r=,P<)。结论美托洛尔与卡维地洛均具有抗动脉粥样硬化的作用,美托洛尔抗动脉粥样硬化的作用呈剂量依赖性;卡维地洛抗动脉粥样硬化的效应优于美托洛尔,其原因可能是卡维地洛具有较强的抗氧化作用。
【关键词】动脉硬化美托洛尔卡维地洛超氧化物歧化酶丙二醛
[ABSTRACT]ObjectiveTo investigate the mechanism of carvedilol and metoprolol in different doses against arterosclerosis pare their therapeutic effect. MethodsMale New Zealand white rabbits (n=40) were randomly and evenly divided into five groups: normal group, highfatdiet group, lowdosemetoprolol group, highdosemetoprolol group and cavedilol group. Ten weeks later, levels of serum superoxide dismutase (SOD) and malondiadehede (MDA) were measured. Expression of PCNA and NFkBp65 in aorta was detected by with the normal group, lower level of serum SOD and higher level of serum MDA were found in the highfatdiet group (F=,