星洲银罗非鱼线粒体DNA的RFLP研究.pdf.pdf

文档介绍：第 28 卷第 3 期广东海洋大学学报
2008 年 6月 Journal of Guangdong Ocean University Jun. 2008

星洲银罗非鱼线粒体 DNA 的 RFLP 研究

刘楚吾 1,黎锦明 1,彭银辉 1,林小坤 2,刘丽 1
( 524025 ,广东湛江 524500)

摘要:用 EcoRⅤ、MboⅡ、NaeⅠ、PvuⅡ、StyⅠ、KpnⅠ、SacⅠ、HindⅢ、DraⅠ、HpaⅡ等 10 种限制性核酸
内切酶对星洲银罗非鱼线粒体 DNA(mtDNA)进行酶切分析,其中 StyⅠ、SacⅠ和 DraⅠ表现出多态性,HpaⅡ酶
切后产生零碎片段,EcoRⅤ、NaeⅠ、PvuⅡ、KpnⅠ只有一个切点,HindⅢ有两个切点,MboⅡ有 5 个切点但有小
片段丢失。在星洲银罗非鱼中共发现 4 种单倍型,单倍型间平均遗传距离为 0,其 mtDNA 多态度为 4,
遗传多样性较丰富。
关键词:星洲银罗非鱼;线粒体 DNA;限制性片段长度的多态性(RFLP)
中图分类号:Q75+ 文献标志码:A 文章编号:1673-9159(2008)03-0016-05

RFLP Analysis on Mitochondrial DNA in Red Tilapia(Oreochromis
niloticus × O. mossambicus)

LIU Chu-wu1, LI Jin-ming1, PENG Yin-hui1, LIN Xiao-kun2, LIU Li1
( College, Guangdong Ocean University, Zhanjiang 524025, China;
2. Fisheries Technology Extension Station of Wuchuan, Zhanjiang 524500, China)

Abstract: The authors analyzed restriction-fragment length polymorphism(RFLP)of mtDNA in red
tilapia(Oreochromis niloticus × O. mossambicus)by 10 restriction endonucleases, EcoRⅤ, MboⅡ,
NaeⅠ, PvuⅡ, StyⅠ, KpnⅠ, SacⅠ, HindⅢ,DraⅠ, HpaⅡ. The result indicated that StyⅠ, SacⅠand
DraⅠ expressed polymorphism and could be sorted into 4 haplotypes. There were many unolderly cut
points by HpaⅡ, which was difficult to analyze; and one cut point by EcoRⅤ, NaeⅠ, PvuⅡ, K