文档介绍：山西医科大学
硕士学位论文
ROCK 抑制剂对脑出血大鼠脑白质损伤的影响
姓名:刘正姣
申请学位级别:硕士
专业:神经病学
指导教师:李东芳
2011-03-20
山西医科大学硕士学位论文

ROCK 抑制剂对脑出血大鼠脑白质损伤的影响

中文摘要

目的:探讨 ROCK 抑制剂---盐酸法舒地尔(Fasudil hydrochloride)对脑出血大鼠神经功能
缺损的保护作用。

方法:运用立体定位仪,取大鼠尾动脉不凝血造内囊出血模型。wistar 雄性清洁级大鼠分
成 3 组:假手术组、脑出血组(ICH 组)、盐酸法舒地尔治疗组。治疗组于出血后 24h 开始
按 12mg/kgd 腹腔注射盐酸法舒地尔液,连续 7d,假手术组和脑出血组用等量的生理盐水
腹腔注射作为对照。运用 Bederson 大鼠神经功能缺损评分方法对各组进行评分。断头取脑
后,行改良三色染色法染色,观察脑组织的病理变化。

结果:盐酸法舒地尔能够明显改善脑出血大鼠的神经功能缺损程度,减轻神经纤维髓鞘脱
失及轴突断裂等病理变化。治疗组血肿周围含水量和神经功能评分均较 ICH 组低(P<),
而较假手术组明显高(P<)。

结论:盐酸法舒地尔可以有效改善脑出血大鼠神经功能缺损情况;盐酸法舒地尔能够减轻
脑出血后血肿周围含水量和降低神经功能评分(Bederson 评分);改良三色染色法染色观
察脑组织的病理变化可以同时显示轴突和髓鞘,是一种更优的神经染色方法。

关键词: 脑出血;ROCK 抑制剂;神经功能缺损;染色

I
山西医科大学硕士学位论文

The effects of Rho-Kinase inhibition on white matter damage after
intracerebral hemorrhage rats
Abstract
Objective: To explore the protective effects of Rho-Kinase inhibition (Fasudil hydrochloride) in
intracerebral hemorrhage rats,whose neurological function were damaged.
Methods: Developing the not coagulation which was taken from the rat tail and injecting it to
the brain internal capsule to establish the model of intracerebral hemorrhage. 250+15g male
Wistar rats were divided into three groups: control group, intracerebral hemorrhage group(ICH
group) and Fasudil hydrochloride treated group. Fasudil hydrochloride was intraperitoneally
injected in the dosage of 12mg/kg/d in treated group from hour 24 after intracerebral hemorrhage,
and followed by 7 days. Rats in control and ICH group were injected with normal saline as
control. Neurological deficit score was evaluated by Bederson ways. Kill the rat and take the
brain after the ess of of improving there-color staining to observe the
brain tissue around the hematoma pathological changes.
Results: Fasudil hydrochloride may significant ameliorate degree of neurological deficit rats,
lessen pathological changes of axon