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洛铂和奥沙利铂对结直肠癌细胞系
生物学行为影响的实验研究
100700 北京北京军区总医院全军普通外科中心
白雪, 于波, 苑树俊, 左富义, 孙亮
【摘要】目的:观察新一代化疗药物洛铂和奥沙利铂对结直肠癌细胞系生物学行为的影响。方法:使用不同浓度的
洛铂与奥沙利铂作用结直肠癌细胞系 LoVo、HR 8348细胞,通过细胞侵袭实验、细胞迁移实验及四唑盐(MTT)比色法来观察
上述细胞在化疗药物作用下生物学行为的变化。结果:洛铂可以显著抑制高转移结肠癌细胞 LoVo的增殖( P < 0 01) ,抑制率
达 60%以上,效果与奥沙利铂相当,同时洛铂亦可以显著抑制 LoVo和直肠癌细胞 HR 8348的侵袭与转移( P < 0 01) ,效果也
与奥沙利铂相当。洛铂对经奥沙利铂作用过的 LoVo细胞仍有较高的抑制率。结论:洛铂作为新一代的铂类化疗药物对结直
肠癌细胞增殖、侵袭、转移均有抑制作用,这为洛铂应用于结直肠癌辅助治疗提供了依据。
【关键词】洛铂; 奥沙利铂; 结直肠癌; 细胞株; 化学治疗
中图分类号: R730 53; R735 3 文献标识码: A 文章编号: 1009 - 0460 (2009) 08 - 0690 - 04
Im pact of colorecta l carc inoma cell line b iolog ica l behav iour by lobapla tin and oxa lipla tin
BA I X ue, YU B o, YUAN Shu jun, ZUO fu yi, SUN L iang. General Surgery Center of PLA, General Hospital of
B m and, B eijing 100700, China
【Abstract】 O bjective: To observe the biological behaviour effect of colorectal carcinoma cell line by lobap latin and oxalip latin.
M ethods: The human colorectal carcinoma cell line LoVo and HR 8348 were used as the research object, and different density lobap la
tin and oxalip latin were as inhibitor. The invasion and metastasis potential were evaluated with transwell chamber. The cell inhibition
ratio was measured with MTT assay. Results:Lobap latin and oxalip latin could make that the abilities of invasion and metastasis were
dropp i