文档介绍：第卷第期载人航天
20 5 Vol. 20 No. 5
年月
2014 9 Manned Spaceflight Sep. 2014
“神舟八号”飞船实时水稻细胞培育及表达谱芯片分析
金晶,陈海莹,蔡伟明∗
中国科学院上海生命科学研究院植物生理生态研究所上海
( , 200032)
摘要:成功建立了在空间微重力条件下水稻细胞的培养体系,并在“神舟八号”飞船空间飞行
时的微重力条件下对培养过程中的细胞进行了实时固定,保存了水稻细胞中反映微重力条件
下基因表达状态的样品。返地后,用质量检测和表达谱芯片方法对回收样品进行
RNA RNA
了分析。提取的水稻细胞质量检测合格,表明本次空间的实验和固定方法可行和可靠。
RNA
进一步分析水稻表达谱变化情况显示,微重力条件下水稻细胞的表达谱相比地面重力条件和
微重力条件下人工条件下的对照有显著差异。
1 g
关键词:神舟八号飞船; 水稻; 细胞;基因;基因芯片;单项匹配实验;整合匹配实验;力学实验
中图分类号: ; 文献标识码: 文章编号: ( )
V524. 1 Q693 A 1674⁃5825 2014 05⁃0480⁃06
Growth of Rice Cells in Shenzhou under
8
Microgravity and Transcriptome Analysis
, , ∗
( JIN Jing CHEN Ha,iying CAI Weiming ,
Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences
, , )
Chinese Academy of Sciences Shanghai 200032 China
Abstract:
Culture system of rice cells under spatial microgravity conditions was essfully estab⁃
,
lished in this study. Moreover a real⁃time fixation of cultured cells was undertaken to get the RNA
samples which reflected the gene expression pattern of rice cells under microgravity conditions. After
,
Shenzhou 8 spaceship returned to earth collected samples were analyzed by RNA quality⁃test and
micro⁃array analysis. The results of RNA quality⁃test indicated that the experimental and fixation
,
methods in space were feasible and reliable. Furthermore analysi