文档介绍：壳寡糖对S180肉瘤细胞凋亡、细胞周期以及凋亡相关蛋白Bcl-2、Bax的影响
【关键词】壳寡糖;S180肉瘤;细胞周期;凋亡;小鼠
摘要:目的探讨壳寡糖对S180肉瘤细胞的生物学效应,为其治疗肿瘤提供新的理论基础。方法体外实验:用流式细胞仪测壳寡糖作用下S180肉瘤细胞周期、细胞凋亡情况。体内实验:建立S180肉瘤小鼠皮下移植瘤模型,给予壳寡糖后,测瘤体积,并用免疫组化法测瘤组织Bax、Bcl-2蛋白的表达情况。结果体外实验:高浓度壳寡糖作用后S180肉瘤细胞阻滞于G 0 .G 1 期,同时S180肉瘤细胞凋亡增加。体内实验:壳寡糖对小鼠S180肉瘤有明显的抑制作用并能提高促凋亡基因Bax的表达,降低抑凋亡基因Bcl-2的表达。结论壳寡糖可抑制S180肉瘤细胞的生长,使S180细胞阻滞于G0 .G 1 期并诱导其发生凋亡,其机制可能与Bcl-2蛋白下调而Bax蛋白上调有关。
关键词:壳寡糖;S180肉瘤;细胞周期;凋亡;小鼠
Abstract:Objective To investigate the biological effect of chito-haride on a180(S180)cells to assess the possibility and potential value of chito-haride in tumor In the experiment in vitro,suspended S180cells were treated with chito- cytometry was used to study the changes in tumor cell apoptosis and cell cycle consequential to the experiment in vivo,Kunming mice bearing S180in groups were given varied doses of chito-haride mice were then killed to determine the tumor
volume and to determine the expression of apoptosis-related proteins(Bcl-2and Bax)by Experiment in vitro showed relatively high concentration of chito-oligosacharride arrested the growth of S180cells at G 0 .G1 phase and increased the apoptotic rate of tumor in vivo showed the growth of S180cells was inhibited by chito-haride(in a concentration-depen-dent manner).The expression of antiapoptotic