文档介绍：食品与药品 Food and Drug 2008 年第 10 卷第 11 期 2 9
正交试验设计优选咽清颗粒的提取工艺
崔晶 1 ,赵宇 2 ,边逸群 2 ,周文 1
(1. 山东大学齐鲁医院药剂科,山东济南 250012;2. 山东大学药学院,山东济南 250012)
摘要:目的通过正交试验设计考察咽清颗粒的提取工艺。方法以总浸膏量和有效成分绿原酸含量为指标,以
溶剂量、提取时间和提取次数为考察因素,每因素设计 3 个水平, 用 L 9 ( 3 4 )正交表安排试验。结果咽清颗
粒的最佳提取工艺为 1 / 3 处方量的药材以 2 00 mL 水回流提取 3 次, 每次 0 . 5 h。结论确定的咽清颗粒提取工艺重
复性好, 操作简单, 具有较高的应用价值。
关键词: 咽清颗粒; 正交试验; 绿原酸; 高效液相色谱法
中图分类号:R282. 71 文献标识码:A 文章编号:1672- 979X( 2008) 11- 0029- 03
Opt i mi zat i on of Ext r act i on Technol ogy of Yanqi ng Gr anul e by Or t hogonal Test
CUI Jing1, ZHAOYu2, BIANYi- qun2, ZHOU Wen1
(1. Depar tment of Pharmacy, Qil u Hospi tal Affi li at ed to Shandong Universit y, Ji nan 250012, Chi na; 2. School
of Phar maceuti cal Sciences, Shandong Univer sit y, Ji nan 250012, China)
Abstr act:Object ive To opt i mi ze t he ext r act i on t echnology of Yanqi ng Gr anul e by ort hogonal t est . Methods
Using the content s of chlorogenic acid and total extract ion as indexes, choosing solvent volume, extract ing durat ion
and extr acting t imes as influential factors, t he opti mal extr acti on condi tion was studied by L9(34) orthogonal test.
Results The opt i mal ext racti on condi ti on of Yanqing Granul e was: 200 mLwat er f or 1/ 3 amount of drugs, r ef l uxing
extraction for 3 times and h each time. Concl usi on The opt imal ext r act i on t echnol ogy obt ai ned in t hi s st udy
i s si