CD4+、CD25+调节性T细胞在重型乙型肝炎发病中的作用.doc

文档介绍：CD4+、CD25+调节性T细胞在重型乙型肝炎发病中的作用
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【摘要】目的探讨CD4+、CD25+调节性T细胞(CD4+、CD25+ Tr)在重型乙型肝炎病人发病机制中的作用。方法采用流式细胞术检测重型乙型肝炎病人及对照组外周血CD4+、CD25+ Tr 的水平及应用PCR测定重型乙型肝炎病人及对照组外周血中HBVDNA滴度。结果重型乙型肝炎组外周血CD4+、CD25+ Tr的百分率为(±)%,较慢性乙型肝炎组的(±)%差异有显著性(),较无症状HBV携带者组及健康对照组差异有非常显著性();×104 copies/ml,较慢性乙型肝炎组(×106 copies/ml)和无症状HBV携带者(×105 copies/ml)差异有非常显著性();不同乙型肝炎病人外周血CD4+、CD25+ Tr的百分率与HBVDNA滴度正相关。结论 CD4+、CD25+ Tr能抑制T细胞对HBV的免疫反应;重型乙型肝炎患者发病与CD4+、CD25+ Tr表达过低有关。
【关键词】 CD4+、CD25+调节性T细胞重型乙型肝炎 CD8+T细胞反应
【Abstract】Objective To explore the possible role of CD4+、CD25+ regulatory T cells in the pathogenesis of severe hepatitis B. Methods The frequency of CD4+、CD25+ Tr in the peripheral blood mononuclear cells (PBMCs) from hepatitis B patients and healthy controls were detected by flow cytometry and their HBVDNA serum levels were detected by PCR. Results 1. A lower proportion of CD4+、CD25+ Tr in PBMCs was found in severe hepatitis B patients(mean%, ± )pared with chronic hepatitis B patients(mean%, ±, ) , chronic asymptomatic HBV carriers() and healthy controls () were significant differences on HBVDNA serum levels of Severe hepatitis B patients(×104 copies/ml) pared with chronic hepatitis B patients( ×106copies/ml,) , chronic asymptomatic HBV carriers (×105co