文档介绍：DNMT1、βcatenin和PGSK3β在结肠癌组织中的表达
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作者:魏永长,贺大林,赵佳辉,白纪蓉
【摘要】目的探讨DNA甲基转移酶1(DNMT1)、β连环素(βcatenin)和磷酸化糖原合成酶激酶(PGSK3β)在结肠癌中的表达及其与肿瘤分化程度和淋巴结转移等的关系。方法采用荧光定量PCR检测62例原发性结肠癌患者癌组织及21例正常大肠黏膜组织中DNMT1、βcatenin和GSK的表达,采用Westernblot方法验证蛋白的表达情况。结果结肠癌组织中DNMT1、βcatenin和PGSK3β表达程度显著高于正常组织。DNMT1在低分化程度的癌组织间相对含量高,βcatenin在肿瘤的不同分化程度及有无淋巴结转移的癌组织间相对含量的差异有统计学意义,而PGSK3β的组间比较无显著性差异。结论 DNMT1、βcatenin和PGSK3β的活性改变与结肠癌有一定的相关性。
【关键词】 DNA甲基转移酶1;磷酸化糖原合成酶激酶;β连环素;结肠癌
ABSTRACT: Objective To examine DNMT1, βcatenin and P
GSK3β expressions in tissues of colon pared with tumor clinicopathological parameters including differentiation and metastasis. Methods SYBR Green realtime PCR and immunoblotting method were used to detect these gene expressions in 62 tissues of colon carcinoma and 21 tissues of normal colon. Results Overexpressions of DNMT1, βcatenin, and PGSK3β were found in colon carcinoma tissues. The expression of DNMT1 was found to be higher in poorly differentiated tissues. The results also demonstrated statistical significance in the expression of βcatenin involving differentiation and metastasis, but no statistical significance in the expression of PGSK3β. Conclusion These results show that DNMT1, βcatenin and PGSK3β expressions are regulated throughout colon cancer progression.
KEY WORDS: DNA methyl transferase 1 (DNMT1); ph