文档介绍：EGFR反义寡核苷酸对人视网膜神经胶质细胞迁移的影响
(作者:___________单位: ___________邮编: ___________)
【摘要】目的:观察表皮生长因子受体(epidermal growth factor receptor,EGFR)反义寡核苷酸(antisense oligonucleotide,ASODN)对人视网膜神经胶质(retinal glial,RG)细胞迁移的影响。方法:人视网膜神经胶质细胞的原代培养,用脂质体(Lipofectin)作为载体将修饰型EGFR ASODN转染RG细胞后,采用Murphy细胞计数方法评价反义寡核苷酸对人RPE细胞体外迁移的影响。结果:脂质体介导EGFR反义寡核苷酸组与对照组比较,细胞迁移活性受到明显抑制(),%。结论:EGFR ASODN能够抑制人RG细胞迁移。
【关键词】视网膜神经胶质细胞;表皮生长因子受体;反义寡核苷酸;脂质体;细胞迁移
AbstractAIM: To observe the effect of antisense oligonucleotide(ASODN) hybridized epidermal growth factor receptor (EGFR) on migration of human retinal glial(RG) cells.METHODS: Human RG cells were cultured in vitro. Modified EGFR ASODN was transfected into RG cells by lipofectin. By using an in vitro wound healing
model in which a small area of a confluent monolayer of RPE cells was denuded,migration was measured by the number of cells that had entered the denuded area.RESULTS: The cell migration was inhibited effectively by ASODN with lipofectin at %.The missense oligonucleotides showed no such effect().CONCLUSION: EGFR ASODN can inhibit the migration of RG cells.
KEYWORDS: retinal glial cell; epidermal growth factor receptor; antisense oligonucleotide; lipofectin; migration
0引言
视网膜胶质( retinal glial,RG)细胞在维持视网膜正常的代谢和功能中起了重要的作用,同时也参与多种病理过程,RG细胞在一些因素的启动下,迁移到玻璃体腔或视网膜表面,增生、形成增生膜并收缩导致一系列病变,在增生性玻璃体视网膜病变(proliferative vitreoretinopathy