文档介绍：HPLCMS法测定人血浆中羧甲司坦浓度
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作者:李婵,傅强,杨建婷,李锋武
【摘要】目的建立一种灵敏、高效、快速的高效液相色谱质谱分析方法,测定人血浆中羧甲司坦的血药浓度。方法 100mL/L高***酸沉淀血浆蛋白,上清液反相高效液相色谱分离,电喷雾离子阱质谱法检测血浆样品中的羧甲司坦,多级反应模式选择离子法定量。,~,%~%。结论该方法精密、灵敏、准确,可用于生物样品中羧甲司坦的测定。
【关键词】羧甲司坦;高效液相色谱离子阱质谱;血药浓度
ABSTRACT: Objective To establish a sensitive, valid and rapid high performance liquid chromatographytrap mass spectrometry (HPLCMS) method for determining carbocisteine concentration in human plasma. Methods Plasma samples were pretreated by 100mL/L perchloric acid to remove the interference protein. The supernatant was separated by reverse phase HPLC, electrospray MS was used to detect carbocisteine in plasma samples, and selective ion method of multiple reaction modes was used to determine the concentration of carbocisteine. Results The limit of detection was
μg/mL and the linear range was -. The recovery rate was within the range of %-%. Conclusion The proposed method could be used to determine carbocisteine in biological samples.
KEY WORDS: carbocisteine; high performance liquid chromatographytrap mass spectrometry; plasma drug concentration
美羧伪麻片是临床广泛应用的一种感冒药,主要成分为羧甲司坦、盐酸伪***和氢溴酸右美沙芬。羧甲司坦具有祛痰作用,能使支气管的黏液分泌减少,痰黏度下降而易被咳出,用于治疗慢性支气管炎、支气管哮喘等症[1]。文献采用高效液相色谱蒸发光检测法或荧光检测法测定其制剂或血浆中羧甲司坦的浓度[24],但方法灵敏度低,特异性不佳。王晓娟等[5]采用柱前在线衍生荧光检测法测定了人