文档介绍：Livin反义寡核苷酸对肺腺癌A549细胞增殖的影响
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作者:宋萍郑金旭管淑红, 邵启祥李坚
【摘要】目的: 探讨Livin基因反义寡核苷酸(antisense oligodeoxynucleotides, ASODN)对肺腺癌A549细胞增殖的影响。方法: 用阳离子脂质体介导Livin ASODN转染至A549细胞,反转录聚合酶链反应(RTPCR)检测Livin基因 mRNA的表达;MTT法检测转染细胞的生长抑制情况。结果: 转染ASODN后A549细胞的Livin 基因mRNA的表达明显减少,Livin 基因ASODN显著抑制A549细胞增殖,并呈剂量依赖性,与对照的错义寡核苷酸(missense oligodeoxynucleotides,MSODN)比较差异有统计学意义()。结论: Livin反义寡核苷酸能明显抑制A549细胞Livin基因 mRNA的表达,抑制细胞增殖,Livin基因有望成为肺癌基因治疗的新靶点。
【关键词】肺癌; 反义寡核苷酸;Livin
[Abstract] Objective: To explore the effects antisense oligodeoxynucleotides(ASODN)of Livin mRNA on the proliferation of human lung adenocarcinoma A549 cells. Methods: Livin ASODN was transfected into A549 cells mediated by lipofectamine 2000. The expressions of Livin mRNA was detected by RTPCR. The proliferation rates of A549 cells were assessed by the MTT assay. Results: It was observed that the expressions of Livin mRNA in ASODN group were decreased in a concentration dependent manner. The cell growth inhibition was evidently increased in the ASODN transfected difference was pared with cells in Livin missense oligodeoxynucleotides (MSODN)(). Conclusion: Livin ASODN significantly down regulated the expression of Livin mRNA and increased growth inhibition in A549 cells. Therefore Livin gene may e a new molecular target for gene therapy for lung cancer.
[Key words] lung