文档介绍：NogoA在脑缺血再灌注白质损伤中的表达及其意义
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作者:高晓玉王得新张拥波
【摘要】目的检测脑缺血再灌注后不同时间点轴突生长抑制因子NogoA在损伤白质区的基因表达,探讨NogoA与缺血再灌注白质损伤的可能关系。方法制备大鼠大脑中动脉缺血再灌注(MCAO)模型,实时定量聚合酶链反应方法(Relative Quantification PCR,RQPCR)检测各时间点NogoA在损伤白质区域的基因表达。结果在损伤白质区域,NogoA基因表达在缺血再灌注1 d时表达量开始减少(与假手术组相比,),7 d时降至最低,14~21 d表达量开始上调,21 d时仍低于假手术组水平(与假手术组相比,)。结论缺血再灌注损伤后白质区域的NogoA基因表达呈现动态变化规律,针对NogoA进行干预有望为缺血再灌注损伤后的脑保护治疗找到新的靶点。
【关键词】脑缺血; NogoA;白质损伤
【Abstract】 Objective To study the expression of NogoA in cerebral white matter after focal cerebral ischemia
reperfusion(I/R) in rats, and explore the relationship between NogoA and cerebral white matter lesions after ischemia. Methods SpragueDawley male rats were subjected to 2 h of middle cerebral artery occlusion (MCAO). Relative quantification PCR(RQPCR) was performed to examine the expressions of NogoA gene at different reperfusion time. Results The expression level of NogoA gene in the white matter was decreased in 1 d group( vs sham group),and was the weakest in 7 d group. It was increased in 14~21 d group, and in 21 d the expression level was still lower than that of sham group(). Conclusions NogoA expreesion has dynamic changes in the white matter after focal cerebral I/R. NogoA could be as one of the targets for neuroprotective therapy after cerebral ischemia.
【Key words】 Cerebral ischemia; N