文档介绍：RNAi沉默EGFR基因对卵巢癌放疗敏感性的影响
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作者:林远洪吴永忠李少林郭启帅罗茜
【摘要】目的探讨表皮生长因子受体(EGFR)基因对卵巢癌放疗敏感性的增强作用。方法构建针对EGFR基因序列特异性短发夹状RNA(shRNA)表达载体,应用卵巢癌SKOV3细胞建立裸鼠移植瘤模型,随机分为对照组、单纯放疗组、基因放疗组,采用6 MV X线照射裸鼠瘤组织,监测瘤体积,测量瘤质量,取瘤组织行RTPCR、Western印迹、免疫组化、电镜检测。结果治疗后三组瘤体积差异均显著(),单纯放疗组、%、%,基因放疗组EGFR基因的表达明显下调,透射电镜下瘤组织凋亡、坏死最明显。结论动物实验证实RNAi沉默EGFR基因明显提高卵巢癌的放疗敏感性。
【关键词】 EGFR基因;RNA干扰;放疗;卵巢癌
【Abstract】 Objective To explore the effects of epidermal growth factor receptor (EGFR) gene on the radiosensitivity of ovarian cancer by RNAi technology. Methods EGFR gene sequencespecific shRNA expression vector were constructed. Tumoranimal model with ovarian cancer SKOV3 cells were established and the nude mice were randomly divided into control, radiotherapy and generadiotherapy groups. The ovarian cancer tissue was irradiated by 6 MV Xray. Tumor volume and tumor weight were measured. RTPCR, Western blot, immunohistochemistry and electron microscopy were used to detected cancer tissue. Results There were obvious difference among three groups in tumor volume (). The inhibitingtumor rate of the radiotherapy and the generadiotherapy groups were % and % respectively. The expression of EGFR gene in generadiotherapy group was significantly decreased and the tumor cell apoptosis, necrosis were all Silenced EGFR gene by RNA