文档介绍：人乳腺癌组织中Wnt/βcatenin信号传导通路的异常表达与Her2阳性表达间的关系
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作者:王珂,任予,何建军,张云锋,陈武科,马清涌
【摘要】目的观察人乳腺癌组织中Wnt/βcatenin信号传导通路主要组分Ecadherin、βcatenin、Cyclin D1的异常表达及其关键组分βcatenin的异常表达与Her2阳性表达之间的关系。方法采用免疫组织化学Elivision两步法检测了90例人乳腺癌组织中Her2、Ecadherin、βcatenin以及Cyclin D1的表达情况。结果 Ecadherin表达正常组中βcatenin的异常表达率(%)显著低于Ecadherin表达缺失组(%);βcatenin异常表达组中Cyclin D1过表达率(%)显著高于βcatenin正常组(%)。Her2阳性组中βcatenin的异常表达率(%)明显高于Her2阴性组(%)。当Her2的阳性表达与βcatenin的异常表达同时存在时腋窝淋巴结转移的阳性率较高。结论在一些人乳腺癌组织中存在Wnt/βcatenin信号传导通路的异常表达,并且其异常表达与Her2的阳性表达有关,二者的异常表达能够协同促进乳腺癌细胞的淋巴结转移。
【关键词】人乳腺癌;Her2;Wnt信号传导通路;E
cadherin;βcatenin;Cyclin D1
: Objective To investigate ectopic expression of key elements of Wnt/βcatenin signaling pathway, including Ecadherin, βcatenin and Cyclin D1, as well as the relationship between ectopic expression of βcatenin and overexpression of Her2 in human breast carcinoma tissues. Methods Immunohistochemical technique (Elivision) was used to detect the expression of Her2 and three elements of Wnt/βcatenin signaling pathway (βcatenin, Ecadherin, Cyclin D1) in 90 samples of human breast carcinoma. Results In Ecadherin normallyexpressed group, the ectopic expression rate of βcatenin (%) was significantly lower than that (%) in Ecadherin negative breast carcinoma; in βcatenin ectopic expression group, overexpression rate of Cyclin D1 (%) was significantly higher than that (%) in the other group. In Her2 overexpressedbreast carcinoma tissue, the ectopic expression rate of βcatenin (%) was significantly higher than that (%) in Her2 negative breast carcinoma. Her2 overexpression could coexist with ectopic expression of Wnt/
βcatenin in promoting axillary lymph node metastasis in human breast carcinoma. Conclusion Ectopic expression of Wnt/βcatenin signaling pathway does exist in some human breast carcinoma tissues, and the abnormal expression correlates with overexpression of Her2. Fu