文档介绍：阿胶对体外培养大鼠成骨细胞增殖、分化功能的影响
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【摘要】目的观察阿胶含药血清对体外培养大鼠成骨细胞增殖、分化功能的影响。方法应用MTT法观察阿胶对体外培养成骨细胞增殖功能的影响,ELISA法观察阿胶对体外培养成骨细胞ALP含量的影响。结果三个剂量组的阿胶含药血清对体外培养大鼠成骨细胞的增殖无促进作用,但对体外培养大鼠成骨细胞内ALP的合成有明显的促进作用。结论阿胶对体外培养大鼠成骨细胞的增殖无促进作用,但能促进体外培养大鼠成骨细胞的分化功能。
【关键词】阿胶;成骨细胞;细胞培养
【Abstract】 Objective To observe the effect of the medicinecontaining blood serum of Ejiao on the multiplicative and differentiative function of the osteoblast of wistar rats cultured in MTT method was used to observe the effect of Ejiao on the multiplicative function of the osteoblast and ELISA metod was adopted to observe the effect of Ejiao on ALP content of the The medicinecontaining blood serum of the three dosage groups of Ejiao had no promotive effect on the multiplicative function of osteoblast, but it had obvious promotive effect on the ALP synthesis inside the Ejiao has no promotive effect on the multiplicative function of the osteoblast, but has differentiative function on the osteoblast of wistar rats cultured in vitro.
【Key words】Chinese medicine; Osteoblast; Cell culture
现代药理研究表明,阿胶(Colla Corii Asini)对血液、造血系统,钙代谢,免疫系统及肿瘤均有明显作用〔1~3〕,且有增强记忆、抗疲劳、耐缺氧等作用〔4,5〕。近年来,有学者开始关注阿胶对骨折愈合的作用,采用原位杂交方法,探讨了阿胶对骨折愈合过程中相关基因表达的影响〔6〕,结果表明,阿胶可加强巨核细胞的聚集及增强其活性,并可促进软骨细胞、成骨细胞的增殖及合成活性,加快软骨内骨化,促进骨愈合。目前,采用血清药理学方法和成骨细胞体外培养技术,观察阿胶对大鼠成骨细胞增殖、分化功能影响的研