文档介绍：高压氧对弥漫性脑损伤大鼠组织病理学的影响
作者:王伯良,曹义战,晋兴,仲月霞,李金声,陆将,何保健,付国强
【关键词】脑损伤
【Abstract】 AIM: To investigate the effect of hyperbaric oxygen on diffuse injured rats brain histopathology. METHODS: We produced headinjured animal model by an weightdropt device designed by Marmarou, the effects of 02 MPa hyperbaric oxygen on brain injury were observed at different time after trauma(6 h, 1 d, 2 d, 3 d, 1 wk, 2 wk and 3 wk). The expression of neurofilament(NF)was observed after trauma using immunohistochemistry methods. The content of water in the rat brain tissue was measured by dried/wet weight method. RESULTS: After trauma, diffuse neuronal degeneration and necrosis were seen and brain water content increased. Axonal rupture was observed by NF staining. But the number of damaged neurons and the brain water content decreased significantly in the HBO group (P<005). CONCLUSION: These results show that diffuse degeneration, necrosis, edema and axonal injury occur after trauma and HBO treatment may decrease the damaged neurons and alleviate brain edema.
【Keywords】hyperbaric oxygenation; brain injuries; brain; pathology; rats
【摘要】目的: 研究高压氧(HBO):采用Marmarou介绍的落体打击装置制作弥漫性脑损伤大鼠模型,观察大鼠脑外伤后6 h,1 d,2 d,3 d,1 wk,2 wk,3 MPa高压氧治疗对其影响,用免疫组化的方法观察损伤脑区NF表达情况,:大鼠脑外伤后顶叶皮层与海马出现不同程度神经元变性坏死;脑组织含水量较正常组高;视束处NF染色可见神经丝蛋白凝集成块,周围有空隙;给予高压氧治疗后坏死神经元较未治疗组明显减少,脑组织含水量明显降低(P<).结论:大鼠脑外伤后出现广泛神经元变性坏死,大面积水肿,弥漫性轴突断裂,而高压氧治疗可明显减轻血管源性脑水肿,改善脑组织供氧,使变性坏死神经元数目减少.
【关键词】高压氧; 脑损伤; 脑; 病理学;大鼠
0 引言
颅脑损伤,特别是重型颅脑损伤患者往往遗留不同程度的神经精神症状和智力障碍,给家庭及社会带来巨大的负担,,高压氧(hyperbaric oxygen, HBO)治疗对颅脑损伤、脑缺血、,我们以大鼠为对象探讨高压氧对弥漫性脑损伤的治疗作用.
1 材料和方法
动物分组选用第四军医大学实验动物中心提供的健康成年雄性 SpragueDawley大鼠75只,体质量350~380 (5只)、损伤组(35只,损伤后6 h, 1 d, 2 d, 3 d, 1 wk, 2 wk, 3 wk, 每个时间点5只)与高压氧治疗组(35只,每个时间点5只).所有动物实验均严格按照有关实验室动物照料及使用规则进行.
动物模型制备采用Marmarou等[1]介绍的动物损伤方法制作弥漫性脑损伤(diffuse brain injury,DBI) g的物体,从150