文档介绍：人宫颈癌组织放射治疗后原癌基因c┐fos的表达
作者:石梅,魏丽春,马海昕,杨守京,陈良为
【关键词】 c-fos
关键词: c-fos;宫颈肿瘤;放射治疗
摘要:目的探讨放疗前、后人宫颈癌组织原癌基因c-fos表达的变化及意义. 方法取临床确诊为宫颈鳞状细胞癌患者的活检组织标本共18例,其中放疗前8例,放疗后10例,在多聚甲醛固定的活检癌组织冰冻切片上,进行Fos蛋白的免疫组织化学染色. 结果放疗前的宫颈癌组织内,多数癌细胞呈Fos免疫染色阳性;照射(30~40Gy)后的宫颈癌组织Fos免疫染色显著降低(P<),多数癌细胞无Fos染色,但放疗前、后间质细胞的Fos免疫反应无明显变化. 结论放射明显抑制宫颈癌细胞的Fos表达,Fos的表达状态可作为放射治疗中评价宫颈癌细胞增殖的分子指标之一.
Keywords:c-fos;cervix neoplasms;radiotherapy
Abstract:AIM To investigate changes of c-fos oncogene ex-pression in the human cervical cancer following irradiation and elucidate its significance in Eighteen cervical cancer biopsy tissues were taken from pa-tients diagnosed with squamous cervical c
ancer,in which ten were treated with radiotherapy;Immunohistochemical stain-ing was performed for Fos protein in formalin-fixed freezing The majority of cancer cells showed
Fos-immunoreactivity(Fos-IR)before irradiation(30~40Gy)Fos-immunoreactivity decreased re-markably(P<),and Fos-immunopositive staining was hardly detected in most of cancer obvious change in Fos-immunoreactivity,however,was seen in mesenchymal connective tissue following Irra-diation inhibits Fos expression in cervical cancer present results also suggest that c-fos oncogene expression may be regarded as a molecular marker for valuing