文档介绍:前列腺酸性磷酸酶在非前列腺腺癌中的表达
作者:王毅,王芳,宿晓云,原田守,山田亮,伊东恭悟,颜炜群
【摘要】目的: 探讨前列腺相关抗原前列腺酸性磷酸酶(PAP)在非前列腺癌中的表达水平. 方法: 分别用RTPCR方法和Western Blot方法检测多种腺癌细胞系中PAP mRNA和PAP蛋白的表达水平;用免疫组织化学方法检测多种腺癌肿瘤组织中的PAP蛋白的表达水平. 结果: 3种结肠癌细胞(colo 201, colo 205和colo 320)、3种胃癌细胞(MKN7, MKN28和 MKN45)和7种乳腺癌细胞(OCUBF, OCUBM, R27, MCF7, CRL1500, YMB1E和MDAMB231)表达PAP mRNA和PAP蛋白,结肠癌、胃癌和乳腺癌肿瘤组织中PAP呈阳性表达. 在检测的5种肾细胞癌细胞和肾细胞癌组织中均未发现有PAP的表达. 结论: PAP是结肠癌、胃癌和乳腺癌的新靶点.
【关键词】酸性磷酸酶;前列腺;腺癌
【Abstract】 AIM: To study the expression of prostatic acid phosphatase (PAP) ,a prostaterelated antigen, in nonprostate cancer cells or tissues. METHODS: A variety of adenocarcinoma cell lines were examined for their PAP expression at the mRNA and protein levels by RTPCR and Western Blot analysis, respectively. The PAP expression in cancer tissues was also examined by immunohistochemical staining. RESULTS: Three kinds of colon cancer cells(colo 201, colo 205 and colo 320), 3 kinds of gastric cancer cells (MKN
7, MKN28 and MKN45), and 7 kinds of breast cancer cell lines (OCUBF, OCUBM,R27, MCF7, CRL1500, YMB1E and MDAMB231) as well as cancer tissues were found to be positive for PAP at both the mRNA and protein levels. But is was not found in 5 kinds of kidney cancer cells or tissues. CONCLUSION: PAP is a new target of nonprostate adenocarcinomas, including colon, breast and gastric cancers.
【Keywords】 acid phosphatase