文档介绍：缺血后处理对高血脂大鼠缺血再灌注心肌Bcl2及Bax蛋白表达的影响
作者:霍玉娥尹博英刘胜辉
【摘要】目的观察缺血后处理对高血脂大鼠缺血再灌注心肌Bcl2及Bax蛋白表达的影响。方法选择高血脂SD大鼠36只,随机分为3组:假手术组、缺血再灌注组、缺血后处理组,每组12只。制备大鼠心肌缺血再灌注模型。缺血再灌注组:收紧结扎线缺血40 min,放松结扎线再灌注240 min;缺血后处理组:缺血40 min后,再灌注10 s,缺血10 s,连续3个循环,然后再灌注240 min;假手术组:开胸后穿线做套环,但不收紧结扎线。再灌注结束后自右颈动脉采血测定血清肌酸激酶(CK)活性,用TUNEL法检测再灌注心肌凋亡程度,采用免疫组织化学方法检测Bcl2及Bax蛋白的表达情况。结果①血清中CK活性的测定:再灌注结束后缺血后处理组和缺血再灌注组CK活性明显高于假手术组〔分别为(±),(±),(±)U/L,P<〕,缺血后处理组明显低于缺血再灌注组(P<)。②心肌凋亡细胞计数:再灌注结束后假手术组未见明显细胞凋亡(<5%),缺血后处理组心肌细胞凋亡率明显低于缺血再灌注组〔分别为(±)%,(±)%,P<〕。③与缺血再灌注组相比,缺血后处理组Bcl2蛋白表达增加(P<),Bax蛋白表达减低(P<)。结论缺血后处理可以增加高血脂大鼠缺血再灌注心肌Bcl2蛋白表达、降低Bax蛋白表达,进而抑制凋亡。
【关键词】再灌注损伤;缺血后处理;凋亡
【Abstract】 Objective To study the effect of ischemic postconditioning on expression of Bcl2 and Bax protein in hyperlipemia rats with myocardial ischemia 36 hyperlipemia SD rats were selected and randomly divided into 3 groups:sham operation,ischemic reperfusion and ischemic postconditioning groups(n=12),and rats were made into MIR in the ischemic reperfusion group were ligated for 40 minutes ischemia,and then reperfused for 240 in ischemic postconditioning group were treated for 40 minutes ischemia,reperfused for 10 s and 10 s ischemia for 3 cycles,and then reperfused reperfussed for 240 in sham operation group were opened the chest to braid for ringer without presence of apoptotic myocytes was detected by(TUNEL). Immunohistochemistry was used to detect the Bcl2 and Bax ,the serum creatine kinase(CK) activity was ① Determination of serum CK activity:it was obviously higher in the ischemic postconditioning group and ischemic reperfusion group after reperfusion than that of the sham operation group〔(±
),(±),(±)U/L respectively, P<〕, and it was significantly lower in the ischemic postconditioning group than that in the ischemic