文档介绍：高效液相色谱法测定降脂减肥颗粒中葛根素含量
【摘要】目的应用高效液相色谱法测定降脂减肥颗粒中葛根素的含量。方法采用Shim-pack-C18柱(5 μm,
150 mm× mm),甲醇-%磷酸(∶)为流动相, mL/min,检测波长为250 nm。~ μg,r= 6,%,RDS=%。结论该方法简便、准确、灵敏,可用于该制剂的质量控制。
【关键词】高效液相色谱法;葛根素;降脂减肥颗粒
Abstract:Objective To determine the content of Puerarin in Jiangzhijianfei Granules by high performance liquid chromatography. Methods The determination was conducted by HPLC using a Shim-pack-C18 column (5 μm, 150 mm× mm) and a mobile phase of methanol-% Phosphoric acid water (∶). The flow rate was mL/min and the detection wavelength was 250 nm. Results Good linear relationship between area and amount was noted for ~ μg of Puerarin, with a correlation coefficient of 6. The average recovery of was %, RSD was %. Conclusion The established method is simple, accurate, sensitive and can be applied to the quality control of the preparation.
Key words:HPLC;Puerarin;Jiangzhijianfei Granules
降脂减肥颗粒是由何首乌、葛根、三七等10味中药组成,具有滋补肝肾、养益精血、扶正固本、通络定痛、健脾豁痰、明目生津、润肠通便的功效,用于各型高血脂症、心脑血管硬化、单纯性肥胖、习惯性便秘、痔疮出血。方中葛根为主要药物。本实验采用高效液相色谱法对制剂中葛根素进行含量测定,以便更好地控制其产品质量。
1 仪器与试药
日本岛津LC-10 AT高效液相色谱仪,SPD-10A紫外检测器,KQ-250型超声波处理器。葛根素对照品购于中国药品生物制品检定所(批号0537-9910,HPLC面积归一化法,%);降脂减肥颗粒(6 g/袋)由吉林森工健今药业有限公司提供。其它化学试剂均为国产,AR级。
2 方法与结果
色谱条件[1]
色谱柱:Diamonsil-C18柱(5 μm, mm×150 mm);流动相为甲醇-%磷酸(
∶)