文档介绍：电离辐射对胶质瘤C6细胞垂体瘤转化基因表达的影响
作者:姜平戴如飞蔡军刘宁骆慧闫志海阎超
【摘要】目的探讨电离辐射对胶质瘤C6细胞垂体瘤转化基因(pituitary tumor transforming gene, PTTG)表达的影响。方法胶质瘤C6细胞分为4组(n=6):A组(空白对照组)、B组(2 Gy剂量X射线组)、C组(4 Gy剂量X射线组)、D 组(6 Gy剂量X射线组),X射线照射后12 h,逆转录聚合酶链反应(RT-PCR)检测PTTG mRNA的表达,免疫细胞化学检测其蛋白表达。结果 PTTG mRNA在X射线处理组均降低,随着X射线剂量的增加,其表达降低越明显,A、B、C、D 4组的表达分别为 ±、 ±、±、±,各组间差异均有统计学意义(P< )。PTTG蛋白积分光密度(integrated optical density,IOD) 在X射线处理组均降低,随着X射线剂量的增加,其表达降低越明显,A、B、C、D ±、±、±、 ±,各组间差异均有统计学意义(P< )。结论电离辐射可以剂量依赖的方式降低胶质瘤C6细胞PTTG mRNA及其蛋白的表达。
【关键词】胶质瘤;电离辐射;垂体瘤转化基因
Abstract: Objective To investigate the effect of ionizing radiation on the expression of pituitary
tumor transforming gene (PTTG) in glioma C6 cells. Methods Glioma C6 cells were divided into 4 groups (n=6): group A (control group), group B (2 Gy doses of X-ray group), group C (4 Gy doses of X-ray group), group D (6 Gy doses of X-ray group). The expression of PTTG mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) 12 hours after X-ray irradiation and the expression of PTTG protein was detected by immunostaining assay using streptavidin-peroxidase (SP) method. Results The expression of PTTG mRNA decreased in X-ray treatment groups, which was positively correlated with the increase of X-ray doses. The expression of PTTG mRNA in groups A, B,