文档介绍：六味地黄丸(浓缩丸)含量测定方法研究
【摘要】目的建立六味地黄丸(浓缩丸)中测定丹皮酚、马钱苷含量的高效液相色谱方法。方法丹皮酚:色谱柱:C18柱(依利特;250 mm× mm,5 μm),流动相:甲醇-水(6∶4), ml·min-1,柱温30℃,检测波长为274 nm。马钱苷:C18柱(依利特;250 mm× mm,5 μm),乙腈-水(15∶85), ml·min-1,柱温25℃,检测波长为236 nm。~ ng范围内呈线性关系,r= 6;%,RSD=%。~ μg范围内呈线性关系,r= 6;%,RSD=%。结论该方法简便,重现性好,可用于六味地黄丸(浓缩丸)中丹皮酚、马钱苷的含量测定。
【关键词】六味地黄丸丹皮酚马钱苷高效液相色谱
Determination of Liuwei Dihuang Pill(concentrated pill)
Abstract:ObjectiveTo establish an HPLC for determination of paeonol and loganin in Liuwei Dihang Pills(concentrated pills).MethodsPaeonol :HPLC column was mobile phase was methanol-water(6∶4) and the flow rate was ml·min- column temperature was at 30
℃ and the detection wavelength was at 274 :HPLC column was mobile phase was acetonitrile-water (15∶85)and the flow rate was ml·min- column temperature was at 25℃ and the detection wavelength was at 236 linear range was from to ng(r= 6) for paeonol and from μg to μg(r= 6) for method is convenient and reproducible for determination of paeonol and loganin in Liuwei Dihuang Pills(concentrated pills)
Key words:Liuwei Dihuang Pills; Paeonol; Loganin; HPLC
六味地黄丸(浓缩丸)质量标准收载于部颁标准第11册。该方出于宋代·钱乙《小儿药证直诀·卷下》,系中医补肾的经典方,为滋阴补肾之著名方剂。笔者参考《中国药典》对该产品进行研究,在原部颁标准的基础上,