文档介绍：轮状病毒性肠炎患儿血清和大便细胞因子的变化
【关键词】轮状病毒
【Abstract】 AIM: To explore the roles of cytokine interleukin8 (IL8) and tumor necrosis factorα(TNFα) in the pathogenesis of rotavirus (RV) enteritis and their clinical significance. METHODS: The levels of IL8 and TNFα in the serum and stool of 46 children with RV enteritis were detected by the doubleantibody sandwich ELISA technique. RESULTS: The levels of IL8 and TNFα in the serum and stool of children with RV enteritis at the acute phase were significantly higher than those in control group (P<), and though they decreased rapidly at the convalescent stage (P<), they were still higher than those in control group (P<). CONCLUSION: IL8 and TNFα are involved in the pathogenesis of RV enteritis.
【Keywords】 rotavirus; enteritis; interleukin8; tumour necrosis factorα
【摘要】目的:探讨细胞因子IL8和TNFα在轮状病毒(RV)肠炎发病中的作用及临床意义. 方法:采用ELISA双抗体夹心法检测46例RV肠炎患儿血清和大便的IL8和TNFα水平. 结果:RV肠炎患儿急性期血清和大便IL8和TNFα水平均显著升高(P<),恢复期血清和大便IL8和TNF
α水平迅速下降(P<),但大便IL8和TNFα水平仍高于对照组(P<). 结论:IL8和TNFα共同参与了RV肠炎的炎症反应.
【关键词】轮状病毒; 肠炎; 白细胞介素8; 肿瘤坏死因子α
0引言
轮状病毒(RV)是婴幼儿秋冬季腹泻的主要病原. RV肠炎的发病机制和免疫机制尚不完全清楚,白细胞介素8(IL8)和肿瘤坏死因子α(TNFα)是体内重要的免疫调节因子和促炎症介质. 我们用ELISA法对46例RV肠炎患儿血清和大便细胞因子IL8和TNFα的水平进行了检测,旨在探讨两者在RV肠炎发病机制中的作用和临床意义.
1对象和方法
200210/12,200310/11 RV肠炎住院患儿46(男28,女18)例,~. 急性期30(男18,女12)例(发病3 d以内),~;恢复期16例(发病4~8 d),临床症状基本消失