文档介绍：:..葡萄籽提取物抗氧化功能实验探究摘要:目的研究葡萄籽提取物的抗氧化功能。方法以SPF级雄性昆明种小鼠为实验动物,根据小鼠体重随机分为5组,每组10只,分别是空白组、模型组和低、中、高3个剂量组,空白组和模型组每天经口给予溶剂植物油,剂量组每天经口给予葡萄籽提取物,连续灌胃30d,取血测抗氧化酶活性。此后除空白对照外,各组射线全身一次性照射5~8Gy60Cov,照射后第4天处死动物,取肝组织测过氧脂质含量和抗氧化酶活性,比较各剂量组与模型组的差别。结果经口给予小鼠葡萄籽提取物30d,高、中剂量组小鼠血中超氧化物歧化酶(SOD)活性,以及高剂量组小鼠血中谷胱甘肽过氧化物酶(GSH-Px)和肝组织中GSH-Px、SOD活性均明显高于模型组,各剂量组小鼠肝中丙二醛(MDA)水平均明显低于模型组。结论葡萄籽提取物有降脂质过氧化的作用,有抗氧化功能。关键词:葡萄籽提取物;抗氧化剂;原花青素中图分类号::A文章编号:1672-979X(2007)11-0016-03EmpiricalStudyonAnti-oxidativeEffectofGrapeSeedExtractFENGDian-juan,XUGui-fa(InstituteofNutritionandFoodHygiene,SchoolofPublicHea1th,ShandongUniversity,Jinan250012,China)Abstract:ObjectiveTostudytheanti-,modelcontroland1ow,moderate,highdosegroups・Innormalcontrolandmodelcontrolgroups,,moderate,highdosegroups,,theactivitiesofGSH-Px,SODinbloodweremeasured・Then,allthegroupsexceptnormalcontrolgroupreceivedone-time5~8Gy60C0Yrayforthewholebodies・Atthefourthdayafterradiation,allthemicewerekilledandtheactivitiesofGSH-Px,,SODactivityinbloodinmoderateandhighdosegroups,andtheactivitiesofGSH-PxinbloodandGSH-Px,SODinhepatictissuesinhighdosegroupwerehigherthanthatinmodelcontrolgroup・Theleve