文档介绍：分子生物学实验常用试剂、缓冲液的配制方法1、1MTris-HCl      □组份浓度1MTris-HCl(,,)  □配制量1L                    □。                                    ,充分搅拌溶解。                                    。                                      pH值              浓HCl                                                      约70mL                                                      约60mL                                                      约42mL                                    。                                    ,室温保存。注意:应使溶液冷却至室温后再调定pH值,因为Tris溶液的pH值随温度的变化差很大,温度每升高1℃,。2、-HCl    □-HCl  ()          □配制量1L                      □。                                  ,充分搅拌溶解。                                  。                                  。                                  ,室温保存。    注意:应使溶液冷却至室温后再调定pH值,因为Tris溶液的pH值随温度的变化差异很大,温度每升高1℃,。3、10×TEBuffer      □组份浓度100mMTris-HCl,10mMEDTA(,,)  □配制量1L                      □,置于1L烧杯中。                                    1MTris-HClBuffer(,,)100mL                                    500mMEDTA()20mL                                  ,均匀混合。                                  ,高温高压灭菌。