文档介绍：Primary study of a Novel Protein F46-crp That Contributes
to Centrosome Duplication1
Wei Yia, Shen Enzhia, Fan Jinlinga, Liu Qiana, Jan Marcb, Wang Yongchaoa, Sun Lec,
Liang Qianjina
a. College of Life Sciences, Beijing Normal University, Beijing, China (100875)
b. School of Biological Sciences, Macleay Building A12, University of Sydney, Sydney 2006,
Australia.
c. Welson Pharmaceuticals, Inc., Haidian, Beijing, China (100085)
E-mail:******@bnu.
Abstract
Using a serum from a patient suffering from an autoimmune disease (progressive systemic sclerosis), a
novel centrosome related protein F46-crp was detected. We identified the target protein by
immunoprecipitation and Western blot and tandem mass spectrometry sequencing. The results show
the protein F46-crp has an apparent molecular mass of about 60 kDa. A cDNA of HeLa F46-crp protein
was obtained using RT-PCR, and a binant F46-crp used to generate monoclonal antibodies.
Immunofluorescence microscopy of synchronized HeLa cells labeled with the anti-F46-crp -specific
antibodies revealed that F46-crp localized exclusively to the centrosome during interphase, although it
completely disappeared at the onset of mitosis. After effectively silencing the F46-crp by antisense
RNA, cell growth and proliferation were strongly inhibited and the cell cycle typically stalled at S
phase. The silencin