文档介绍：29(3)469—472中国生物防治学报ChineseJournalofBiologicalControl2013年8月BtCrylAb原核表达产物与苹果蠹蛾颗粒体病毒协同增效初探吕婷婷,徐兵强2,,王敦(,杨凌712100:,乌鲁木齐830091)摘要:利用基因工程表达的BtCrylAb毒素蛋白,表达菌裂解后粗产物以非致毒的微量水平添加苹果蠹蛾颗粒体病毒喀什株1(CypoGV-KS1)作为生物增效剂,测定添加不同比例的BtCrylAb毒素蛋白对CypoGV-KS1毒力的增进效果。结果表明:微量BtCrylAb的添加能够显著缩短CypoGV-KS1对苹果蠹蛾的致死时间。当CypoGV-×10PIBs·mL-、·mL时,即可产生显著的增效作用,%%;·mL1时,%%,达到极显著水平差异。关健词:CypoGV-KS1;BtCrylAb;增效作用;苹果蠹蛾中图分类号:$:A文章编号:1005—9261(2013)03—0469—04TheEnhancementonCydiapomonellaGranulovirusVirulencebyProkaryoticExpressedBtCrylAbLOTingting,XUBingqiang2,ABUDUKEYOUMUKader2,WANGDun’,MinistryofEducation/NorthwestA&FUniversity,Yangling712100;,XinjiangAcademyofAgricultureScience,Wulumuqi830091)Abstract:TheprokaryoticexpressedBtCrylAbproteinwasusedasavirulenceenhancerforCydiapomonellagranulovirusKashgarstrain1(CypoGV-KS1)-KS1suspensionatdifferentlevelsandthe【T5oand【-%%,respect