文档介绍：UV-VIS
GC
HPLC
FTIR
AES AAS MS
EMMISSION
ELECTROCHEM
INSTRUMENTAL ANALYSIS
e-COURSE NOTE
TEXTBOOKS:
●Instrumental Analysis,Zhu Ming-hua,Higher
Education Press(3rd)(Textbook for 21th Century),
Beijing, 2001(in Chinese)
● Modern Analytical Chemistry, David Harvey,
McGraw-Hill, Inter. Ed., Singapore, 2000
● Contemporary Instrumental Analysis, ,
. Rubinson, Science Press & Prentice Hall, Inc.,
Beijing, 2000(影印版)
HOW to study this course
●熟悉仪器分析课程各章节的编排规律,各种方法的
意义及特点、各种分析方法的联系和区别。
●充分发挥学生的能动作用,做到师生互动;反馈渠道通畅。
●协调好课堂学习与课后复习、课前预习的关系。
●遵守课堂秩序和课堂纪律
Teaching Purpose
●仪器分析是化学与化学工程类专业必修的基础课之一。
通过本课程的学习
●要求学生掌握: Principle of instrumental analytical methods mon use and simple setup;
●要求学生初步具有 An ability to develop appropriate analytical procedure according to the analytical purpose based on the advantage and application fields of different instrumental analyses studied.
Chapter 1 PREFACE
Description of Validation Parameters
Accuracy Closeness of agreement between the value obtained by the method and the true value.
Precision Expresses the closeness of agreement between a series of
measurements obtained from multiple sampling. Precision is often
expressed as the standard deviation or Relative standard deviation of replicate measurements (later on RSD). Note that a method can be precise, but not accurate (so all your measurements may be close together, but the result is wrong).
Specificity The ability to measure the analyte in the presence ponents which we expect to be present in the sample matrix. So if you are determining the concentration of Iron and Chromium in water by UV/Vis spectroscopy, if there are small amounts of Fluoride and Chloride in the sample, will that affect your measurement?
Detection Limit The lowest amount of analyte in a sample that can be detected, but not necessarily quantitated. Usually the lowest limit is evaluated as the Signal-to-Noise ratio that is equivalent to 3 times the standard deviation of the no